Biofilm forming rhizobacteria enhance growth and salt tolerance in sunflower plants by stimulating antioxidant enzymes activity

• Published in: Plant physiology and biochemistry 2020-11, Vol.156, p.242-256
• Main Authors: Yasmeen, Tahira, Ahmad, Aqeel, Arif, Muhammad Saleem, Mubin, Muhammad, Rehman, Khadija, Shahzad, Sher Muhammad, Iqbal, Shahid, Rizwan, Muhammad, Ali, Shafaqat, Alyemeni, Mohammed Nasser, Wijaya, Leonard
• Format: Article
• Language: English
• Subjects: Antioxidants - metabolism; Biofilms; Helianthus - microbiology; Helianthus - physiology; Nutrient uptake; Oxidative stress; PGPR; Photosynthesis; Plant Roots; Rhizobiaceae - physiology; Salinity tolerance; Salt Tolerance; Soil Microbiology
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2020.09.016

Salinity stress is one of the major environmental stresses that impose global socio-economic impacts, as well as hindering crop productivity. Halotolerant plant growth-promoting rhizobacteria (PGPR) having potential to cope with salinity stress can be employed to counter this issue in eco-friendly way. In the present investigation, halotolerant PGPR strains, AP6 and PB5, were isolated from saline soil and characterized for their biochemical, molecular and physiological traits. Sequencing of 16 S rRNA gene and comparative analysis confirmed the taxonomic affiliation of AP6 with Bacillus licheniformis and PB5 with Pseudomonas plecoglossicida. The study was carried out in pots with different levels of induced soil salinity viz. 0, 5, 10 and 15 dSm−1 to evaluate the potential of bacterial inoculants in counteracting salinity stress in sunflower at different plant growth stages (30, 45 and 60 days after sowing). Both the bacterial inoculants were capable of producing indole acetic acid and biofilm, solubilizing inorganic rock phosphate, and also expressed ACC deaminase activity. The PGPR inoculated plants showed significantly higher fresh and dry biomass, plant height, root length and yield plant−1. Ameliorative significance of applied bacterial inoculants was also evidenced by mitigating oxidative stress through upregulation of catalase (CAT), superoxide dismutase (SOD) and guaiacol peroxidase (GPX) antioxidant enzymes. Increase in photosynthetic pigments, gas exchange activities and nutrient uptake are crucial salt stress adaptations, which were enhanced with the inoculation of salt tolerant biofilm producing PGPR in sunflower plants. Although increase in salinity stress levels has gradually decreased the plant's output compared to non-salinized plants, the plants inoculated with PGPR confronted salinity stress in much better way than uninoculated plants. Owing to the wide action spectrum of these bacterial inoculants, it was concluded that these biofilm PGPR could serve as effective bioinoculants and salinity stress alleviator for sunflower (oil seed crop) by increasing crop productivity in marginalized agricultural systems. [Display omitted] •Biofilm forming PGPR inoculant approach was developed to improve sunflower salt tolerance.•Biofilm PGPR solubilized P, secreted IAA, and expressed ACC-deaminase activity.•PGPR mediated salt tolerance in sunflower was evident by oxidative stress reduction.•Plant physiological and biochemical functions at highest salinit