OsRbohB-mediated ROS production plays a crucial role in drought stress tolerance of rice

• Published in: Plant cell reports 2020-12, Vol.39 (12), p.1767-1784
• Main Authors: Shi, Yi, Chang, Yan-Li, Wu, Hai-Tao, Shalmani, Abdullah, Liu, Wen-Ting, Li, Wen-Qiang, Xu, Jian-Wei, Chen, Kun-Ming
• Format: Article
• Language: English
• Subjects: Abscisic acid; Abscisic Acid - metabolism; Biomedical and Life Sciences; Biotechnology; Cell Biology; Cellular stress response; Dehydration; Developmental stages; Drought; Drought resistance; Droughts; Gene Expression Regulation, Plant; Gene Knockout Techniques; Gene regulation; Genes; Germination; Homology; Life Sciences; Mutants; NAD(P)H oxidase; NADPH Oxidases - genetics; NADPH Oxidases - metabolism; Original Article; Oryza - genetics; Oryza - metabolism; Oryza - physiology; Oxidase; Plant Biochemistry; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Sciences; Plants, Genetically Modified; Reactive Oxygen Species - metabolism; Respiratory burst oxidase; Rice; Seed germination; Signaling; Stomata; Stress, Physiological - physiology; Transcription; Transgenic plants
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/s00299-020-02603-2

Key message We found that a rice NADPH oxidase gene OsRbohB contributes drought tolerance and its functions are involved in the interaction of the OsRbohB-mediated ROS production and ABA signaling. The plasma membrane NADPH oxidases, also known as respiratory burst oxidase homologs, are the key producers of ROS under both normal and stress conditions in plants. However, their functions in rice development and stress tolerance are still under investigation. Here, we found that a rice NADPH oxidase gene OsRbohB , also named OsNOX1 , is expressed in all tissues examined throughout the development stages with higher transcripts in leaves. The transcriptional expression of OsRbohB is also strongly stimulated by dehydration, salt and several phytohormonal treatments. Compared with wide-type and the OsRbohB -overexpressing transgenic plants, osrbohB , a Tos17 insertion knockout mutant of OsRbohB , shows lower ROS production, abscisic acid (ABA) content and transcripts of a series of stress-related genes. The osrbohB mutant also exhibits lower seed germination rate, organ size and thousand seed weight, but higher stomatal aperture and sensitivity to drought. Moreover, a number of genes involved in plant development, stress response, transcriptional regulation, and particularly ABA signaling are differentially expressed in osrbohB plants under both normal growth and drought conditions. All these results suggest the roles of OsRbohB in drought tolerance of rice, which probably performed through the interaction of the OsRbohB-mediated ROS production and ABA signaling.