Electrochemical immunosensor based on binary nanoparticles decorated rGO-TEPA as magnetic capture and Au@PtNPs as probe for CEA detection

Using gold and magnetic nanoparticles co-decorated reduced graphene oxide-tetraethylenepentamine (rGO-TEPA/Au-MNPs) as the magnetic platform for capturing the primary antibody (Ab 1 ), separation and preconcentration of immunocomplex, a novel homogeneous electrochemical immunosensor was successfully...

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Bibliographic Details
Published in:Mikrochimica acta (1966) 2020-10, Vol.187 (10), p.584-584, Article 584
Main Authors: Cao, Liangli, Xiao, Haolin, Fang, Cheng, Zhao, Feijun, Chen, Zhencheng
Format: Article
Language:English
Subjects:
Analytical Chemistry
Antibodies
Antigens
Biomarkers
CEA (Oncology)
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Comparative analysis
Electrical conductivity
Electrical resistivity
Graphene
Immunoassay
Immunosensors
Microengineering
Nanochemistry
Nanoparticles
Nanotechnology
Original Paper
Selectivity
Stability analysis
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Summary:Using gold and magnetic nanoparticles co-decorated reduced graphene oxide-tetraethylenepentamine (rGO-TEPA/Au-MNPs) as the magnetic platform for capturing the primary antibody (Ab 1 ), separation and preconcentration of immunocomplex, a novel homogeneous electrochemical immunosensor was successfully developed. The newly prepared magnetic rGO-TEPA/Au-MNPs, compared with MNPs, exhibited better stability and enhanced electrical conductivity attributed to rGO-TEPA, and showed higher biorecognition efficiency due to AuNPs. In addition, Au@PtNPs were prepared and modified with secondary antibody (Ab 2 ) as an efficient signal probe for signal readout. Using carcinoembryonic antigen (CEA) as a model analyte, the prepared immunosensor demonstrated satisfactory properties like high stability, good repeatability and selectivity, wide linear range (5.0 pg mL −1 ~200.0 ng mL −1 ) as well as low detection limit (1.42 pg mL −1 ). The homogenous electrochemical immunosensor was applied to the detection of CEA in human serum and was found to exhibit good correlation with the reference method. Thus, the proposed rGO-TEPA/Au-MNPs-based homogenous immunoassay platform might open up a new way for biomarker diagnosis. Graphical Abstract
ISSN:0026-3672
1436-5073
DOI:10.1007/s00604-020-04559-2