Quantitative proteomic profiling of targeted proteins associated with Loki Zupa Decoction Treatment in OVA-Induced asthmatic mice

• Published in: Journal of ethnopharmacology 2021-02, Vol.266, p.113343-113343, Article 113343
• Main Authors: Wuniqiemu, Tulake, Qin, Jingjing, Teng, Fangzhou, Nabijan, Mohammadtursun, Cui, Jie, Yi, La, Tang, Weifeng, Zhu, Xueyi, Abduwaki, Muhammadjan, Nurahmat, Mammat, Wei, Ying, Dong, Jing cheng
• Format: Article
• Language: English
• Subjects: Airway inflammation; Airway remodeling; Animals; Anti-Asthmatic Agents - isolation & purification; Anti-Asthmatic Agents - pharmacology; Anti-Inflammatory Agents - isolation & purification; Anti-Inflammatory Agents - pharmacology; Asthma; Asthma - drug therapy; Cytokines - metabolism; Disease Models, Animal; Drugs, Chinese Herbal - pharmacology; Female; Inflammation - drug therapy; Inflammation - pathology; Loki zupa (LKZP) decoction; Medicine, Chinese Traditional - methods; Mice; Mice, Inbred BALB C; Ovalbumin; Parallel reaction monitoring (PRM); Proteomics; Quantitative proteomics; Receptors, Cell Surface - metabolism; Traditional Chinese medicine (TCM)
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2020.113343

Loki Zupa (LKZP) decoction is one of the herbal prescriptions in traditional Uyghur medicine, which is commonly used for treating airway abnormality. However, underlying pathological mechanism and pathways involved has not been well studied. In this paper, we aim to further confirmed the anti-inflammatory and anti-fibrotic role of LKZP decoction in airway, and uncover the passible mechanism involved via comprehensive quantitative proteomic DIA-MS analysis. Mice asthmatic model was established with sensitizing and challenging with OVA. Lung function, pathological status, and inflammatory cytokines were assessed. Total of nine lung tissues were analyzed using proteomic DIA-MS analysis and 18 lung tissues were subjected to PRM validation. Total of 704 differentially expressed proteins (DEPs) (363 up regulated, 341 down regulated) were quantified in comparison of asthmatic and healthy mice, while 152 DEPs (91 up regulated, 61 down regulated) were quantified in LKZP decoction treated compared to asthmatic mice. Total of 21 proteins were overlapped between three groups. ECM-receptor interaction was significantly enriched and commonly shared between downregulated DEPs in asthma and upregulated DEPs in LKZP decoction treated mice. Total of 20 proteins were subjected to parallel reaction monitoring (PRM) analysis and 16 of which were quantified. At last, two proteins, RMB 10 and COL6A6, were validated with significant difference (P