A cytosolically localized far-red to near-infrared rhodamine-based fluorescent probe for calcium ions

Ca 2+ is one of the most important second messengers in cells. A far-red to near-infrared (NIR) Ca 2+ fluorescent probe is useful for multi-color imaging in GFP or YFP-expressing biosamples. Here we developed a cytosolically localized far-red to NIR rhodamine-based fluorescent probe for Ca 2+ , CaSi...

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Bibliographic Details
Published in:Analyst (London) 2020-11, Vol.145 (23), p.7736-774
Main Authors: Numasawa, Koji, Hanaoka, Kenjiro, Ikeno, Takayuki, Echizen, Honami, Ishikawa, Tomoe, Morimoto, Masakazu, Komatsu, Toru, Ueno, Tasuku, Ikegaya, Yuji, Nagano, Tetsuo, Urano, Yasuteru
Format: Article
Language:English
Subjects:
Calcium
Calcium ions
Fluorescent Dyes
Fluorescent indicators
Ions
Lysosomes
Mitochondria
Near infrared radiation
Rhodamine
Rhodamines
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Summary:Ca 2+ is one of the most important second messengers in cells. A far-red to near-infrared (NIR) Ca 2+ fluorescent probe is useful for multi-color imaging in GFP or YFP-expressing biosamples. Here we developed a cytosolically localized far-red to NIR rhodamine-based fluorescent probe for Ca 2+ , CaSiR-2 AM , while rhodamine dyes are basically localized to mitochondria or lysosomes in cells. We developed the cytosolically localized far-red to NIR fluorescent probe for Ca 2+ , CaSiR-2 AM , utilizing the rhodamine scaffold.
ISSN:0003-2654
1364-5528
DOI:10.1039/d0an01739f