Bioactive extracts from brewer's spent grain

In this study antioxidant-rich extracts from brewer’s spent grain (BSG) extracted with solid-to-liquid extraction using different solvents water and ethanol and their mixtures at two ratios (80% ethanol:water (v/v) and 60% ethanol:water (v/v)) were characterized. Nutritional composition was evaluate...

Full description

Saved in:
Bibliographic Details
Published in:Food & function 2020-10, Vol.11 (1), p.8963-8977
Main Authors: Bonifácio-Lopes, Teresa, Vilas Boas, Ana, Coscueta, Ezequiel, Costa, Eduardo, Silva, Sara, Campos, Débora, Teixeira, José, Pintado, Maria Manuela
Format: Article
Language:English
Subjects:
Antibacterial activity
Antihypertensive
Antihypertensives
Antioxidant activity
Antioxidants
Bioactive compounds
Bioactivity
Biocompatibility
Biological activity
Biological properties
Brewer’s spent grain
Catechin
Cytotoxicity
Deoxyribonucleic acid
DNA
Ethanol
Genotoxicity
High-performance liquid chromatography
Liquid chromatography
Phenolic compounds
Phenols
Solvents
Toxicity
Vanillin
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this study antioxidant-rich extracts from brewer’s spent grain (BSG) extracted with solid-to-liquid extraction using different solvents water and ethanol and their mixtures at two ratios (80% ethanol:water (v/v) and 60% ethanol:water (v/v)) were characterized. Nutritional composition was evaluated for the extracts and for the solid residues obtained after extraction. Additionally, the extracts were analyzed for the total phenolic content and individual phenolic compounds and related biological properties including antioxidant capacity (ABTS; ORAC and DNA protection), antihypertensive capacity, antibacterial activity and antibiofilm capacity. Safety was also demonstrated through genotoxicity and cytotoxicity tests. The results obtained showed that while all the extracts exhibited high antioxidant capacity (except ethanolic extract), the highest values were obtained for the 60% ethanol:water extract. The identification of phenolic compounds using HPLC showed that catechin and vanillin were the main compounds identified with the highest concentration being obtained for 60% ethanol:water extraction. In the biological activity assays water and hydroethanolic extracts showed to be multifunctional (antioxidant and antihypertensive capacity, antibacterial and antibiofilm activity), the 80% ethanol:water presented better results in some assays. All were non-genotoxic, but the cytotoxicity was dependent on the extract concentration, with complete safe application for all up to 1 mg/mL. Therefore, this study shows the potential of a viable green solvent based and a low cost extraction recovery method of bioactive compounds from brewer’s spent grain.
ISSN:2042-6496
2042-650X
DOI:10.1039/d0fo01426e