Relationship between bovine oocytes developmental competence and mRNA expression of apoptotic and mitochondrial genes following the change of vitrification temperatures and cryoprotectant concentrations

• Published in: Cryobiology 2020-12, Vol.97, p.110-122
• Main Authors: Zhang, Zhi Yang, Yu, Xue Li, Cai, Meng Dan, Liu, Yi Heng, Liu, Jia Qi, Zhao, Shi Yu, Li, Xiao Xia, Li, Ying Hua
• Format: Article
• Language: English
• Subjects: Animals; Apoptotic gene; Cattle; Cryopreservation - methods; Cryoprotective agent concentrations; Developmental competence; Genes, Mitochondrial; Mitochondrial gene; mRNA expression; Oocyte; Oocytes; RNA, Messenger - genetics; Temperature; Vitrification; Vitrification temperatures
• ISSN: 0011-2240; 1090-2392
• DOI: 10.1016/j.cryobiol.2020.09.009

The present study analyzed the relationship between bovine oocytes developmental competence and mRNA expression of apoptotic and mitochondrial genes following the change of vitrification temperatures (VTs) and cryoprotectant agent concentrations (CPAs). Cumulus oocyte complexes were randomly divided into five groups: control, vitrified in liquid nitrogen (LN; −196 °C) with 5.6 M CPAs (LN 5.6 M), LN with 6.6 M CPAs (LN 6.6 M), liquid helium (LHe; −269 °C) with 5.6 M CPAs (LHe 5.6 M), and LHe with 6.6 M CPAs (LHe 6.6 M). After vitrification and warming, oocytes of vitrified and control groups were subjected to in vitro maturation (IVM), in vitro fertilization and in vitro culture. The blastocyst rate in LHe 5.6 M group was the highest among the four vitrified groups (13.7% vs. 9.4%, 1.3%, and 8.4%; P