Multifunctional bilateral muscle control of vocal output in the songbird syrinx

• Published in: Journal of neurophysiology 2020-12, Vol.124 (6), p.1857-1874
• Main Authors: Méndez, Jorge M, Goller, Franz
• Format: Article
• Language: English
• ISSN: 0022-3077; 1522-1598
• DOI: 10.1152/jn.00332.2020

Songbirds produce complex vocalizations by coordinating neuromuscular control of syrinx, respiratory system, and upper vocal tract. The functional roles of syringeal muscles have been documented mainly with correlative data, which have suggested that synergistic activation plays a role in the fine control of vocal features. However, the specific involvement of individual muscles in achieving this fine control is still largely unknown. Here we investigate the contributions of the two main airflow controlling muscles, the dorsal and ventral tracheobronchial muscles in the zebra finch, through a new approach. Ablation of the muscle insertion on the cartilage framework reveals detailed insights into their respective roles in the fine control of song features. Unilateral ablation of a tracheobronchial muscle resulted in mostly subtle changes of the air sac pressure pattern and song features. Effects of ablation varied with the acoustic elements, thus indicating a context-dependent specific synergistic activation of muscles. High-frequency notes were most affected by the ablation, highlighting the importance of coordinated bilateral control. More pronounced effects on song features and air sac pressure were observed after bilateral ablation of the dorsal tracheobronchial muscles. The results illustrate that the gating muscles serve multiple functions in control of acoustic features and that each feature arises through context-dependent, synergistic activation patterns of syringeal muscles. Although many changes after the ablation are subtle, they fall within the perceptual range and thus may control behaviorally relevant features of sound. These data therefore provide important specific details about the underlying motor code for song production. A new experimental approach was used to analyze the involvement of individual muscles in birdsong vocal control. Ablation of tracheobronchial muscles showed how these muscles contribute in manner specific to the acoustic structure of sound segments and how disruption of airflow regulation affects bilateral coordination. The results of this study illustrate that the gating muscles serve multiple functions in control of acoustic features and give further insight into the complex motor control of birdsong.