Regeneration of Rat Sciatic Nerve Using PLGA Conduit Containing Rat ADSCs with Controlled Release of BDNF and Gold Nanoparticles

Implantation of a nerve guidance conduit (NGC) carrying neuroprotective factors is promising for repairing peripheral nerve injury. Here, we developed a novel strategy for repairing peripheral nerve injury by gold nanoparticles (AuNPs) and brain-derived neurotrophic factor (BDNF)–encapsulated chitos...

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Bibliographic Details
Published in:Journal of molecular neuroscience 2021-04, Vol.71 (4), p.746-760
Main Authors: Jahromi, Maliheh, Razavi, Shahnaz, Seyedebrahimi, Reihaneh, Reisi, Parham, Kazemi, Mohammad
Format: Article
Language:English
Subjects:
Alginates
Alginic acid
Biomedical and Life Sciences
Biomedicine
Brain-derived neurotrophic factor
Cell Biology
Chitosan
Controlled release
Encapsulation
Gold
Histology
Immunohistochemistry
Laminin
Nanofibers
Nanoparticles
Neurochemistry
Neurology
Neuroprotection
Neurosciences
Peripheral nerves
Polylactide-co-glycolide
Proteomics
Regeneration
Sciatic nerve
Stem cell transplantation
Stem cells
Surgical implants
Transplantation
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Summary:Implantation of a nerve guidance conduit (NGC) carrying neuroprotective factors is promising for repairing peripheral nerve injury. Here, we developed a novel strategy for repairing peripheral nerve injury by gold nanoparticles (AuNPs) and brain-derived neurotrophic factor (BDNF)–encapsulated chitosan in laminin-coated nanofiber of Poly(l-lactide-co-glycolide) (PLGA) conduit and transplantation of rat adipose–derived stem cells (r-ADSCs) suspended in alginate. Then, the beneficial effect of AuNPs, BDNF, and r-ADSCs on nerve regeneration was evaluated in rat sciatic nerve transection model. In vivo experiments showed that the combination of AuNPs- and BDNF-encapsulated chitosan nanoparticles in laminin-coated nanofiber of PLGA conduit with r-ADSCs could synergistically facilitate nerve regeneration. Furthermore, the in vivo histology, immunohistochemistry, and behavioral results demonstrated that the AuNPs- and BDNF-encapsulated chitosan nanoparticles in NGC could significantly reinforce the repair performance of r-ADSCs, which may also contribute to the therapeutic outcome of the AuNPs, BDNF, and r-ADSCs strategies. In this study, we found that the combination of AuNPs and BDNF releases in NGC with r-ADSCs may represent a new potential strategy for peripheral nerve regeneration.
ISSN:0895-8696
1559-1166
DOI:10.1007/s12031-020-01694-6