Optimization of triacetate cellulose hollow fiber vitrification (HFV) method for cryopreservation of in vitro matured bovine oocytes

The aim of the current work was to evaluate applicability of triacetate cellulose hollow fiber vitrification (HFV) method for cryopreservation of groups of in vitro matured bovine oocytes (12–17 oocytes per device). We also attempted to optimize HFV protocol by altering concentration of non-permeati...

Full description

Saved in:
Bibliographic Details
Published in:Cryobiology 2020-12, Vol.97, p.66-70
Main Authors: Kornienko, Ekaterina V., Romanova, Anastasia B., Ikonopistseva, Maria V., Malenko, Galina P.
Format: Article
Language:English
Subjects:
Animals
Blastocyst
Bovine
Cattle
Cellulose
Cryopreservation
Cryopreservation - methods
Cryoprotective Agents - pharmacology
Fertilization in Vitro
Hollow fiber
Oocyte
Oocytes
Vitrification
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The aim of the current work was to evaluate applicability of triacetate cellulose hollow fiber vitrification (HFV) method for cryopreservation of groups of in vitro matured bovine oocytes (12–17 oocytes per device). We also attempted to optimize HFV protocol by altering concentration of non-permeating cryoprotectant (sucrose) in vitrification solution and concentration of extracellular Ca2+ by using a calcium-free base medium for preparation of vitrification/rewarming solutions with ethylene glycol (EG) as a single permeating cryoprotectant. Neither of modifications of HFV protocol significantly affected survival or fertilization rates of the vitrified bovine oocytes. Embryo development rates in the vitrification groups were lower than those in the control (31.2% of blastocysts at Day 8 post IVF). Use of vitrification/rewarming solutions with lower Ca2+ concentration and EG did not significantly improve embryo development rates. An increase of sucrose concentration in vitrification solution from 0.5 to 1.0 M significantly improved blastocyst yield on Day 8 post IVF (21.1–23.4% vs 3.1–3.5%; p 
ISSN:0011-2240
1090-2392
DOI:10.1016/j.cryobiol.2020.10.007