Isopentylamine is a novel defence compound induced by insect feeding in rice

• Published in: Plant, cell and environment cell and environment, 2021-01, Vol.44 (1), p.247-256
• Main Authors: Aboshi, Takako, Iitsuka, Chiaki, Galis, Ivan, Teraishi, Masayoshi, Kamo, Marina, Nishimura, Ayami, Ishihara, Atsushi, Mori, Naoki, Murayama, Tetsuya
• Format: Article
• Language: English
• Subjects: Accumulation; amine; Amines; Amino acids; Aquatic plants; Biosynthesis; defence; Feeding; Herbivores; Herbivory; Insects; Jasmonic acid; Labeling; Leaves; Leucine; Metabolites; Nilaparvata lugens; Plant hormones; Polyamines; Rice; rice (Oryza sativa); secondary metabolism; Seedlings; Stable isotopes
• ISSN: 0140-7791; 1365-3040
• DOI: 10.1111/pce.13902

Plants produce a broad variety of defensive metabolites to protect themselves against herbivorous insects. Although polyamines have been implicated in various responses to abiotic and biotic stress, there have been no studies focused on amines in response to insect herbivory. By screening for bioactive amines, we identified isopentylamine as a novel type of herbivory‐induced compound in rice leaves, which was derived from the amino acid leucine in stable isotope labelling experiments. Accumulation of isopentylamine increased during herbivory by the brown planthopper (Nilaparvata lugens, BPH) and the rice‐feeding armyworm (Mythimna loreyi), as well as in response to treatment with the plant hormone, jasmonic acid. Likewise, isopentylamine accumulation was compromised in rice jasmonate biosynthesis mutants, hebiba and Osjar1. In bio‐assays, BPH insects feeding on rice seedlings submerged in 50 mg/L isopentylamine solution had a higher mortality compared with BPH feeding on seedlings submerged in water. Notably, the rice leaves submerged in 50 mg/L solution showed the endogenous concentrations of isopentylamine similar to that induced by BPHs. These results suggest that isopentylamine functions as a new type of plant defence metabolite that is rapidly induced by herbivore attack and deters insect herbivores in rice. We identified isopentylamine as a novel type of herbivory‐induced compound in rice leave. The bioassay results using the brown planthopper suggest that isopentylamine functions as a new type of plant defence metabolite that deters insect herbivores in rice.