Colorimetric and fluorescent dual-identification of glutathione based on its inhibition on the 3D ball-flower shaped Cu-hemin-MOF’s peroxidase-like activity

Hemin as the organic linker ligand and Cu (II) as the metal center were applied to prepare a copper-metal-organic framework (Cu-hemin-MOF) via one-step hydrothermal method. Characterization using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron...

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Bibliographic Details
Published in:Mikrochimica acta (1966) 2020-11, Vol.187 (11), p.601-601, Article 601
Main Authors: Chen, Xiaolong, Wang, Xianfeng, Cao, Gaihua, Wu, Yawen, Luo, Huibo, Ji, Zhong, Shen, Caihong, Huo, Danqun, Hou, Changjun
Format: Article
Language:English
Subjects:
Analytical Chemistry
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Colorimetry
Colorimetry - instrumentation
Colorimetry - methods
Copper
Copper - chemistry
Diffraction
Fluorescence
Fourier transforms
Glutathione
Glutathione - chemistry
Hemin - chemistry
Hydrogen peroxide
Infrared spectroscopy
Metal-organic frameworks
Microengineering
Molecular Structure
Nanochemistry
Nanotechnology
Organometallic Compounds - chemistry
Original Paper
Peroxidase
Phenylenediamine
Photoelectrons
Spectrum analysis
Substrates
Three dimensional flow
Ultraviolet radiation
X ray photoelectron spectroscopy
X ray powder diffraction
X-ray spectroscopy
X-rays
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Summary:Hemin as the organic linker ligand and Cu (II) as the metal center were applied to prepare a copper-metal-organic framework (Cu-hemin-MOF) via one-step hydrothermal method. Characterization using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and X-ray powder diffraction (XRD) demonstrate that the acquired Cu-hemin-MOF possesses the appearance of 3D ball-flower shape with the existence of C, N, O, Fe, and Cu on the surface. Further study found that this 3D ball-flower shaped Cu-hemin-MOF exhibits peroxidase-like activity, which can catalyze the peroxidase substrate of o-phenylenediamine (OPD) to generate 2,3-diaminophenazine (DAP) in the presence of H 2 O 2 . DAP has a yellow color and also emits a strong fluorescence when excited by ultraviolet light. Interestingly, Cu-hemin-MOF’s peroxidase-like activity can be strongly inhibited by glutathione (GSH). In view of this, a dual readout (fluorescence and colorimetry) was proposed to detect GSH for the first time. Under optimal conditions, the proposed method exhibits good linear relationship between the signal response (fluorescence and colorimetry) and the concentration of GSH, and low limits of detection (LOD) of 2.3 and 26.6 nM, respectively. Graphical abstract
ISSN:0026-3672
1436-5073
DOI:10.1007/s00604-020-04565-4