Regeneration of lingual musculature in rats using myoblasts over porcine bladder acellular matrix

Objectives To use tissue engineering muscle repair (TEMR) for regenerating the lingual musculature of hemiglossectomized rats using neonatal myoblasts (NM) on porcine acellular urinary bladder matrix (AUBM). Material and Methods The study used 80 male rats. A volumetric muscle loss (VML) injury was...

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Bibliographic Details
Published in:Oral diseases 2021-09, Vol.27 (6), p.1580-1589
Main Authors: Camacho‐Alonso, Fabio, García‐Carrillo, Nuria, Buendía, Antonio Julián, Navarro, Jose Antonio, Peñarrocha‐Oltra, David, Peñarrocha‐Diago, Miguel, Lacal‐Luján, Jose
Format: Article
Language:English
Subjects:
Dentistry
Desmin
hemiglossectomized tongues
Morphometry
Myoblasts
neonatal myoblasts
Neonates
porcine acellular urinary bladder matrix
Regeneration
Tissue engineering
tissue engineering muscle repair
Tongue
Urinary bladder
volumetric muscle loss
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Summary:Objectives To use tissue engineering muscle repair (TEMR) for regenerating the lingual musculature of hemiglossectomized rats using neonatal myoblasts (NM) on porcine acellular urinary bladder matrix (AUBM). Material and Methods The study used 80 male rats. A volumetric muscle loss (VML) injury was created on the left side of the tongue. The rats were randomized into four groups: Group 1 (AUBM + myoblasts); Group 2 (AUBM); Group 3 (myoblasts); and Group 4 (control). NM were obtained from neonatal rats. The animals were weighed on day 0 and just before euthanasia. Five rats in each group were euthanized at days 2, 14, 28, and 42; the tongues were prepared for morphometric analysis, postoperative left hemitongue weight, and immunohistochemical analysis (desmin, CD‐31, and anti‐neurofilament). Results The weight gain from greatest to least was as follows: AUBM + myoblasts > myoblasts > AUBM > control. The tongue dorsum occupied by VML, and difference in mg between control side and intervened side from least to great was as follows: AUBM + myoblasts  control. Conclusion The use of porcine AUBM and NM for the regeneration of lingual musculature was found to be an effective TEMR treatment for repairing tongue VML injury.
ISSN:1354-523X
1601-0825
DOI:10.1111/odi.13674