Application of Scanning Electron Microscopy in the observation of dentin‐adhesive interface

The objective of this study was observation of the adhesive interface on original tooth samples, as well as their epoxy replicas, under SEM. A light‐cure flowable composite was incrementally placed and light‐polymerized in previously prepared cylindrical dentinal cavities on the buccal surfaces of e...

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Bibliographic Details
Published in:Microscopy research and technique 2021-04, Vol.84 (4), p.602-607
Main Authors: Ramić, Bojana D., Stojanac, Igor Lj, Drobac, Milan R., Kantardžić, Ivana R., Maletin, Aleksandra Z., Cvjetićanin, Milica T., Otašević, Katarina S., Petrović, Ljubomir M.
Format: Article
Language:English
Subjects:
Adhesive bonding
adhesive interface
Adhesives
composites
Dental caries
Dentin
dentin bonding
Micrography
Molars
Morphology
Photomicrographs
Polymerization
Scanning electron microscopy
Teeth
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Summary:The objective of this study was observation of the adhesive interface on original tooth samples, as well as their epoxy replicas, under SEM. A light‐cure flowable composite was incrementally placed and light‐polymerized in previously prepared cylindrical dentinal cavities on the buccal surfaces of extracted human third molars. After finishing procedures, impressions of the composite/dentin margin were made using polyvinylsiloxane in order to obtain accurate epoxy replicas for SEM analysis. Ultrastructural morphology of the adhesive surface was observed at high magnifications (≥1,000×) on original tooth samples, which were previously prepared to expose the part of the dentin surface, which participates in the formation of adhesive bond. SEM micrographs showed that marginal adaptation was mostly of acceptable quality. In some of the SEM micrographs of original tooth samples, marginal gap formation, and resin tag breakdown were noted, which were ascribed to polymerization shrinkage. Profound understanding of ultrastructural morphology is necessary for achieving more predictable and durable margin between composite restorations and surrounding tooth structures, and SEM analysis can serve that purpose. In this paper, we report on the influence of the application of scanning electron microscopy in the observation of ultrastructural morphology of the adhesive interface in achieving more predictable margin between composite restorations and surrounding tooth structures.
ISSN:1059-910X
1097-0029
DOI:10.1002/jemt.23618