A loop-mediated isothermal amplification (LAMP) assay for detection of Toxoplasma gondii infection in women with spontaneous abortion

The present study aimed to use the loop-mediated isothermal amplification (LAMP) technique in comparison with serological tests to determine the rate of  T. gondii infection in women suffering from spontaneous abortion (SA). A total of 140 women suffering from their first SA were included in this st...

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Bibliographic Details
Published in:Archives of microbiology 2021-03, Vol.203 (2), p.763-769
Main Authors: Kheirandish, Farnaz, Fallahi, Shirzad, Mahmoudvand, Hossein, Araban, Ali, Anbari, Khatereh, Rouzbahani, Arian Karimi, Akbari, Soheila
Format: Article
Language:English
Subjects:
Abortion, Spontaneous - etiology
Adult
Antibodies
Biochemistry
Biomedical and Life Sciences
Biotechnology
Cell Biology
Ecology
Enzyme-Linked Immunosorbent Assay
Female
Fetuses
Frequency distribution
Gene amplification
Humans
IgG antibody
Immunoglobulin G
Immunoglobulin G - blood
Immunoglobulin M
Immunoglobulin M - blood
Infections
Life Sciences
Microbial Ecology
Microbiology
Miscarriage
Molecular Diagnostic Techniques
Nucleic Acid Amplification Techniques
Original Paper
Parasitology - methods
Pregnancy
Serological tests
Serology
Statistical analysis
Statistical methods
Toxoplasma
Toxoplasma - immunology
Toxoplasmosis - complications
Toxoplasmosis - diagnosis
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Summary:The present study aimed to use the loop-mediated isothermal amplification (LAMP) technique in comparison with serological tests to determine the rate of  T. gondii infection in women suffering from spontaneous abortion (SA). A total of 140 women suffering from their first SA were included in this study. The collected aborted fetal remains and blood samples from each patient were examined in sterilized conditions using the LAMP technique and ELISA. Of the 140 women, 80 (57.1%) tested seropositive for anti- Toxoplasma antibodies by ELISA, 72 (51.4%) women tested seropositive for the IgG antibody, 8 (5.7%) tested seropositive for the IgM antibody. Among the eight women who’d had their first SA who tested seropositive for IgM antibody by ELISA, only five cases (62.5%) reported positively to the LAMP test. The difference in the frequency distribution of the LAMP results for measuring the Toxoplasma infection in pregnant women under study was statistically significant ( P  
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-020-02081-w