Diet during early life defines testicular lipid content and sperm quality in adulthood

• Published in: American journal of physiology: endocrinology and metabolism 2020-12, Vol.319 (6), p.E1061-E1073
• Main Authors: Crisóstomo, Luís, Videira, Romeu A, Jarak, Ivana, Starčević, Kristina, Mašek, Tomislav, Rato, Luís, Raposo, João F, Batterham, Rachel L, Oliveira, Pedro F, Alves, Marco G
• Format: Article
• Language: English
• Subjects: Age Factors; Animal Nutritional Physiological Phenomena; Animals; Animals, Newborn; Antioxidants; Body weight loss; Carbohydrates; Children; Damage; Diet; Diet, High-Fat - adverse effects; Fatty acids; High carbohydrate diet; High fat diet; Inflammation; Lipid metabolism; Lipid Metabolism - physiology; Lipids; Lipids - analysis; Male; Metabolic disorders; Metabolic syndrome; Metabolism; Metabolites; Mice; Mitochondria; NMR; Nuclear magnetic resonance; Obesity; Obesity - complications; Obesity - metabolism; Obesity - pathology; Parameters; Polyunsaturated fatty acids; Reproductive health; Reproductive system; Semen Analysis; Sexual Maturation - physiology; Sperm; Spermatozoa - physiology; Testes; Testis - chemistry; Testis - metabolism; Testis - pathology; Time Factors; Weaning; Weight control; Weight loss
• ISSN: 0193-1849; 1522-1555
• DOI: 10.1152/AJPENDO.00235.2020

Childhood obesity is a serious concern associated with ill health later in life. Emerging data suggest that obesity has long-term adverse effects upon male sexual and reproductive health, but few studies have addressed this issue. We hypothesized that exposure to high-fat diet during early life alters testicular lipid content and metabolism, leading to permanent damage to sperm parameters. After weaning ( after birth), 36 male mice were randomly divided into three groups and fed with a different diet regimen for 200 days: a standard chow diet (CTRL), a high-fat diet (HFD) (carbohydrate: 35.7%, protein: 20.5%, and fat: 36.0%), and a high-fat diet for 60 days, then replaced by standard chow (HFD ). Biometric and metabolic data were monitored. Animals were then euthanized, and tissues were collected. Epididymal sperm parameters and endocrine parameters were evaluated. Testicular metabolites were extracted and characterized by 1H-NMR and GC-MS. Testicular mitochondrial and antioxidant activity were evaluated. Our results show that mice fed with a high-fat diet, even if only until early adulthood, had lower sperm viability and motility, and higher incidence of head and tail defects. Although diet reversion with weight loss during adulthood prevents the progression of metabolic syndrome, testicular content in fatty acids is irreversibly affected. Excessive fat intake promoted an overaccumulation of proinflammatory n-6 polyunsaturated fatty acids in the testis, which is strongly correlated with negative effects upon sperm quality. Therefore, the adoption of high-fat diets during early life correlates with irreversible changes in testicular lipid content and metabolism, which are related to permanent damage to sperm quality later in life.