Continuous expression and functional prediction of circular RNA in mouse lung development

To study the continuous expression and potential function of circular RNA (circRNA), circ4:150439343|150477468 and circ15:73330849|73343359, in mouse lung development. According to the stage of lung development, lung tissue samples were collected from mice on embryonic day 16.5 (E16.5), embryonic da...

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Published in:Zhongguo dang dai er ke za zhi 2020-10, Vol.22 (10), p.1125-1130
Main Authors: Fu, Xue, Yang, Yang, Shen, Yan-Qing, Zhou, Xiao-Guang, Zhou, Xiao-Yu
Format: Article
Language:Chinese
Subjects:
Animals
Lung
Mice
MicroRNAs - genetics
Phosphatidylinositol 3-Kinases
Real-Time Polymerase Chain Reaction
RNA, Circular - genetics
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Summary:To study the continuous expression and potential function of circular RNA (circRNA), circ4:150439343|150477468 and circ15:73330849|73343359, in mouse lung development. According to the stage of lung development, lung tissue samples were collected from mice on embryonic day 16.5 (E16.5), embryonic day 18.5 (E18.5), and postnatal day 2 (P2). Hematoxylin and eosin staining was performed to observe the morphology of lung tissue. Quantitative real-time PCR (qRT-PCR) was used to measure the mRNA expression of circ4:150439343|150477468 and circ15:73330849|73343359 during late lung development; miRanda and TargetScan were used to predict the target miRNAs of circRNAs, and then GO and KEGG analysis was performed for the target genes to predict the potential function of circRNAs. Type II alveolar epithelial cells were observed in the lung slices of E16.5 mice, with a gradual increase in number. On P2, the pulmonary alveoli expanded rapidly, the pulmonary interstitium became thinner, and the alveolar structure gradually
ISSN:1008-8830
DOI:10.7499/j.issn.1008-8830.2004071