Galactomannan of Delonix regia seeds reduces nociception and morphological damage in the rat model of osteoarthritis induced by sodium monoiodoacetate

• Published in: Naunyn-Schmiedeberg's archives of pharmacology 2021-03, Vol.394 (3), p.491-501
• Main Authors: da Silva Nascimento, Francisco Glerison, de Souza Ferreira Bringel, Pedro Henrique, Maia, Francisco Wildson Silva, Lima, Carlos Pinheiro Chagas, Alves, Rômulo Couto, Feitosa, Judith Pessoa Andrade, Mota, Mário Rogério Lima, Assreuy, Ana Maria Sampaio, Castro, Rondinelle Ribeiro
• Format: Article
• Language: English
• Subjects: Analgesics - therapeutic use; Animals; Arthritis; Biomedical and Life Sciences; Biomedicine; Delonix regia; Disease Models, Animal; Ethanol; Fabaceae; Foot Joints - drug effects; Foot Joints - pathology; Galactose; Galactose - analogs & derivatives; Iodoacetic Acid; Male; Mannans - therapeutic use; Mannose; Neurosciences; Nociception - drug effects; Original Article; Osteoarthritis; Osteoarthritis - chemically induced; Osteoarthritis - drug therapy; Osteoarthritis - pathology; Pain - chemically induced; Pain - drug therapy; Pain - pathology; Pain perception; Pharmacology/Toxicology; Rats; Rats, Wistar; Seeds
• ISSN: 0028-1298; 1432-1912
• DOI: 10.1007/s00210-020-01996-x

This study investigated the effects of the protein-free galactomannan obtained from Delonix regia seeds (GM-DR) in an experimental osteoarthritis (OA) model. GM-DR was obtained from water-homogenized endosperms by collection of the supernatant and precipitation with ethanol. The remaining proteins in the galactomannan were removed by alkaline hydrolysis. Weight average molar mass ( M w ) of the galactomannan was estimated in 5.8 × 10 5 g mol −1 , presenting mannose:galactose ratio of 2.39:1. Rats received sodium monoiodoacetate (OA groups, 1 mg/25 μL) or saline (sham group) in the right tibio-tarsal joint. GM-DR (30–300 μg) was administered by intra-articular route at days 14 and 21 after OA induction. Hypernociception was evaluated daily by the measurement of the mechanical threshold required to cause joint flexion and paw withdrawal reflex. The 56-day animal groups were euthanized for joint histopahological analysis using the OARSI score system. Lower doses of GM-DR (30 and 100 μg) promoted antinociception from day 15 until the endpoint at day 56. Joint damage was reduced by GM-DR administration (100 μg) in OA-subjected animals, compared to the vehicle-treated OA group (5.9 ± 1.8 vs 19.0 ± 1.8, respectively, p < 0.05). Conclusion: Both antinociception and damage reduction suggest that Delonix regia galactomannan is a promising approach for osteoarthritis therapy.