Lactobacillus casei CRL431 modulates hemostatic activation induced by protein malnourishment and pneumococcal respiratory infection

• Published in: Applied microbiology and biotechnology 2020-12, Vol.104 (24), p.10669-10683
• Main Authors: Zelaya, Hortensia, Laiño, Jonathan, Villena, Julio, Marranzino, Gabriela, Alvarez, Susana, Agüero, Graciela
• Format: Article
• Language: English
• Subjects: Animals; Applied Microbial and Cell Physiology; Bacteria; Biomedical and Life Sciences; Biotechnology; Coagulants; Coagulation; Complications and side effects; Control; Diet; Dietary supplements; Fibrin; Functional foods & nutraceuticals; Hemostasis; Hemostatics; Immunomodulation; Immunoregulation; Inflammation; Influence; Lactobacilli; Lactobacillus; Lactobacillus casei; Life Sciences; Lungs; Malnutrition; Mice; Microbial Genetics and Genomics; Microbiology; P-selectin; Platelets; Pneumococcal Infections; Pneumonia, Pneumococcal; Probiotics; Proteins; Respiratory tract infection; Respiratory Tract Infections; Streptococcus infections; Streptococcus pneumoniae; Tissue factor
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-020-10957-6

Previously, we demonstrated that Lactobacillus casei CRL431, a well-known immunomodulatory bacterium, beneficially regulates coagulation activation, fibrin formation in lung, and the pro-inflammatory state induced by protein malnourishment and pneumococcal infection. In this study, we deepen in the understanding of the mechanisms involved in the immunoregulatory activity of L. casei CRL431 during a nutritional repletion process by evaluating (a) platelet and endothelial activation, (b) tissue factor (TF) expression, and (c) protease-activated receptor (PAR) activation in an experimental bacterial respiratory infection model in malnourished mice. Our findings demonstrate for the first time that the repletion diet supplemented with L. casei CRL431 was effective to normalize platelet counts in blood, modulate platelet activation and their recruitment into the lung, and regulate local and systemic TF expression and endothelial activation, which were affected by malnourishment. Streptococcus pneumoniae challenge induced local and systemic increase of platelet counts, PARs activation, P-selectin and TF expression, as well as endothelial activation in both well-nourished and malnourished mice. Malnourished animals evidenced the highest alterations of the parameters evaluated while the mice fed with the probiotic bacterium had similar behavior to normal controls but with lower PAR activation in lung. These results demonstrate that supplementation of repletion diet with L. casei CRL431 is effective to modulate alterations induced by malnourishment and pneumococcal infection, restraining coagulation activation, the inflammatory process, and lung damage. These observations contribute to set the basis for the application of probiotic functional foods to modulate the inflammation–hemostasis interactions altered by malnourishment or bacterial respiratory infections. Key points • Pneumococcal infection increases pro-coagulant state induced by protein malnourishment. • Repletion with L. casei CRL431 modulates platelet, TF, and endothelial activation. • L. casei CRL431 improves immune-coagulative response in protein malnourishment.