Ribonucleases control distinct traits of Pseudomonas putida lifestyle

Summary The role of archetypal ribonucleases (RNases) in the physiology and stress endurance of the soil bacterium and metabolic engineering platform Pseudomonas putida KT2440 has been inspected. To this end, variants of this strain lacking each of the most important RNases were constructed. Each mu...

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Bibliographic Details
Published in:Environmental microbiology 2021-01, Vol.23 (1), p.174-189
Main Authors: Apura, Patrícia, Lorenzo, Víctor, Arraiano, Cecília M., Martínez‐García, Esteban, Viegas, Sandra C.
Format: Article
Language:English
Subjects:
Adaptation
E coli
Ecosystem
Endoribonucleases - genetics
Endoribonucleases - metabolism
Escherichia coli - metabolism
Exoribonucleases - genetics
Exoribonucleases - metabolism
Metabolic engineering
Metabolism
Microorganisms
Morphology
Oxidation-Reduction
Physiological responses
Physiology
Polynucleotide phosphorylase
Pseudomonas putida
Pseudomonas putida - genetics
Pseudomonas putida - metabolism
Ribonuclease E
Ribonuclease G
Ribonuclease III
Soil
Soil bacteria
Soil Microbiology
Soil microorganisms
Soil stresses
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Summary:Summary The role of archetypal ribonucleases (RNases) in the physiology and stress endurance of the soil bacterium and metabolic engineering platform Pseudomonas putida KT2440 has been inspected. To this end, variants of this strain lacking each of the most important RNases were constructed. Each mutant lacked either one exoribonuclease (PNPase, RNase R) or one endoribonuclease (RNase E, RNase III, RNase G). The global physiological and metabolic costs of the absence of each of these enzymes were then analysed in terms of growth, motility and morphology. The effects of different oxidative chemicals that mimic the stresses endured by this microorganism in its natural habitats were studied as well. The results highlighted that each ribonuclease is specifically related with different traits of the environmental lifestyle that distinctively characterizes this microorganism. Interestingly, the physiological responses of P. putida to the absence of each enzyme diverged significantly from those known previously in Escherichia coli. This exposed not only species‐specific regulatory functions for otherwise known RNase activities but also expanded the panoply of post‐transcriptional adaptation devices that P. putida can make use of for facing hostile environments.
ISSN:1462-2912
1462-2920
DOI:10.1111/1462-2920.15291