Vesicular glutamate transporter‐immunopositive axons that coexpress neuropeptides in the rat and human dental pulp

• Published in: International endodontic journal 2021-03, Vol.54 (3), p.377-387
• Main Authors: Cho, Y. S., Ko, H. G., Han, H. M., Park, S. K., Moozhayil, S. J., Choi, S. Y., Bae, Y. C.
• Format: Article
• Language: English
• Subjects: Animals; Axons; Calcitonin; Calcitonin gene-related peptide; Dental Pulp; Dentistry; Ganglia; glutamate; Glutamic acid transporter; Humans; Immunohistochemistry; Inflammation; inflammatory pain; Kruskal-Wallis test; Maxilla; neuropeptide; Neuropeptides; Orthodontics; Pain; Parvalbumin; Rats; Rats, Sprague-Dawley; Substance P; Teeth; Trigeminal ganglion; Vesicular Glutamate Transport Proteins; vesicular glutamate transporter
• ISSN: 0143-2885; 1365-2591
• DOI: 10.1111/iej.13427

Aim To examine the type of vesicular glutamate transporter (VGLUT)‐immunopositive (+) axons that coexpress neuropeptides in the rat and human dental pulp, which may help understand peripheral mechanism of pulpal inflammatory pain in rats and humans. Methodology The trigeminal ganglia (TG) and the dental pulp of the maxillary molar teeth from three male Sprague–Dawley rats weighing 300–330 g and dental pulps of three healthy human (male) maxillary premolar teeth from three 16 to 28‐year‐old patients extracted for orthodontic treatment were used. The type of VGLUT + axons that coexpress substance P (SP)‐ and/or calcitonin gene‐related peptide (CGRP) and parvalbumin in the rat TG and in the axons of the rat and the human dental pulp was examined by double fluorescence immunohistochemistry and quantitative analysis. Results were analyzed using one‐way anova and the Kruskal–Wallis test. Results SP and CGRP were expressed in many human VGLUT1 + pulpal axons but not in the rat VGLUT1 + TG neurons and pulpal axons (P