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Vesicular glutamate transporter‐immunopositive axons that coexpress neuropeptides in the rat and human dental pulp
Aim To examine the type of vesicular glutamate transporter (VGLUT)‐immunopositive (+) axons that coexpress neuropeptides in the rat and human dental pulp, which may help understand peripheral mechanism of pulpal inflammatory pain in rats and humans. Methodology The trigeminal ganglia (TG) and the de...
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| Published in: | International endodontic journal 2021-03, Vol.54 (3), p.377-387 |
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| container_issue | 3 |
| container_start_page | 377 |
| container_title | International endodontic journal |
| container_volume | 54 |
| creator | Cho, Y. S. Ko, H. G. Han, H. M. Park, S. K. Moozhayil, S. J. Choi, S. Y. Bae, Y. C. |
| description | Aim
To examine the type of vesicular glutamate transporter (VGLUT)‐immunopositive (+) axons that coexpress neuropeptides in the rat and human dental pulp, which may help understand peripheral mechanism of pulpal inflammatory pain in rats and humans.
Methodology
The trigeminal ganglia (TG) and the dental pulp of the maxillary molar teeth from three male Sprague–Dawley rats weighing 300–330 g and dental pulps of three healthy human (male) maxillary premolar teeth from three 16 to 28‐year‐old patients extracted for orthodontic treatment were used. The type of VGLUT + axons that coexpress substance P (SP)‐ and/or calcitonin gene‐related peptide (CGRP) and parvalbumin in the rat TG and in the axons of the rat and the human dental pulp was examined by double fluorescence immunohistochemistry and quantitative analysis. Results were analyzed using one‐way anova and the Kruskal–Wallis test.
Results
SP and CGRP were expressed in many human VGLUT1 + pulpal axons but not in the rat VGLUT1 + TG neurons and pulpal axons (P |
| doi_str_mv | 10.1111/iej.13427 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2453689558</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2453689558</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3537-77f8fd40630c1d8c9cfec36c9d729ad6afb3395499a990639c26af35680efd53</originalsourceid><addsrcrecordid>eNp10c9qFTEUBvAgir1WF76ABNzYxbTJnMlMspTS1kqhm9LtkCZnbC4zyZg_td31EXxGn8TorS4EswkkPz4O5yPkLWeHvJ4jh9tDDl07PCMbDr1oWqH4c7JhvIOmlVLskVcpbRljggF_SfYAmGKdhA3J15icKbOO9Mtcsl50Rpqj9mkNMWP88fjdLUvxYQ3JZXeHVN8Hn2i-1ZmagPdrxJSoxxLDimt2FhN1vv4jjZVob-ltWbSnFn3WM13LvL4mLyY9J3zzdO-Tq9OTq-NPzcXl2fnxx4vGgIChGYZJTrZjPTDDrTTKTGigN8oOrdK219MNgBKdUlqpqpRp6xuIXjKcrIB98mEXu8bwtWDK4-KSwXnWHkNJY9sJ6KUSQlb6_h-6DSX6OlxVUvKuBwlVHeyUiSGliNO4Rrfo-DByNv5qYqxNjL-bqPbdU2K5WdD-lX9WX8HRDnxzMz78P2k8P_m8i_wJiCeVfA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2488146383</pqid></control><display><type>article</type><title>Vesicular glutamate transporter‐immunopositive axons that coexpress neuropeptides in the rat and human dental pulp</title><source>Wiley</source><creator>Cho, Y. S. ; Ko, H. G. ; Han, H. M. ; Park, S. K. ; Moozhayil, S. J. ; Choi, S. Y. ; Bae, Y. C.</creator><creatorcontrib>Cho, Y. S. ; Ko, H. G. ; Han, H. M. ; Park, S. K. ; Moozhayil, S. J. ; Choi, S. Y. ; Bae, Y. C.</creatorcontrib><description>Aim
To examine the type of vesicular glutamate transporter (VGLUT)‐immunopositive (+) axons that coexpress neuropeptides in the rat and human dental pulp, which may help understand peripheral mechanism of pulpal inflammatory pain in rats and humans.
Methodology
The trigeminal ganglia (TG) and the dental pulp of the maxillary molar teeth from three male Sprague–Dawley rats weighing 300–330 g and dental pulps of three healthy human (male) maxillary premolar teeth from three 16 to 28‐year‐old patients extracted for orthodontic treatment were used. The type of VGLUT + axons that coexpress substance P (SP)‐ and/or calcitonin gene‐related peptide (CGRP) and parvalbumin in the rat TG and in the axons of the rat and the human dental pulp was examined by double fluorescence immunohistochemistry and quantitative analysis. Results were analyzed using one‐way anova and the Kruskal–Wallis test.
Results
SP and CGRP were expressed in many human VGLUT1 + pulpal axons but not in the rat VGLUT1 + TG neurons and pulpal axons (P < 0.05). SP and CGRP were expressed in a considerable number of human VGLUT2 + pulpal axons and also in many rat TG neurons and pulpal axons. The fraction of VGLUT1 + axons expressing parvalbumin was about three times higher in the rat than in the human dental pulp (P < 0.05).
Conclusions
These findings suggest that the types of VGLUT + axons, which release neuropeptides, may be different between the rat and the human dental pulp, raising a possibility that peripheral mechanism of pulpal inflammatory pain may be different between rats and humans.</description><identifier>ISSN: 0143-2885</identifier><identifier>EISSN: 1365-2591</identifier><identifier>DOI: 10.1111/iej.13427</identifier><identifier>PMID: 33090483</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Animals ; Axons ; Calcitonin ; Calcitonin gene-related peptide ; Dental Pulp ; Dentistry ; Ganglia ; glutamate ; Glutamic acid transporter ; Humans ; Immunohistochemistry ; Inflammation ; inflammatory pain ; Kruskal-Wallis test ; Maxilla ; neuropeptide ; Neuropeptides ; Orthodontics ; Pain ; Parvalbumin ; Rats ; Rats, Sprague-Dawley ; Substance P ; Teeth ; Trigeminal ganglion ; Vesicular Glutamate Transport Proteins ; vesicular glutamate transporter</subject><ispartof>International endodontic journal, 2021-03, Vol.54 (3), p.377-387</ispartof><rights>2020 International Endodontic Journal. Published by John Wiley & Sons Ltd</rights><rights>2020 International Endodontic Journal. Published by John Wiley & Sons Ltd.</rights><rights>Copyright © 2021 International Endodontic Journal. Published by John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3537-77f8fd40630c1d8c9cfec36c9d729ad6afb3395499a990639c26af35680efd53</citedby><cites>FETCH-LOGICAL-c3537-77f8fd40630c1d8c9cfec36c9d729ad6afb3395499a990639c26af35680efd53</cites><orcidid>0000-0002-7618-418X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33090483$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cho, Y. S.</creatorcontrib><creatorcontrib>Ko, H. G.</creatorcontrib><creatorcontrib>Han, H. M.</creatorcontrib><creatorcontrib>Park, S. K.</creatorcontrib><creatorcontrib>Moozhayil, S. J.</creatorcontrib><creatorcontrib>Choi, S. Y.</creatorcontrib><creatorcontrib>Bae, Y. C.</creatorcontrib><title>Vesicular glutamate transporter‐immunopositive axons that coexpress neuropeptides in the rat and human dental pulp</title><title>International endodontic journal</title><addtitle>Int Endod J</addtitle><description>Aim
To examine the type of vesicular glutamate transporter (VGLUT)‐immunopositive (+) axons that coexpress neuropeptides in the rat and human dental pulp, which may help understand peripheral mechanism of pulpal inflammatory pain in rats and humans.
Methodology
The trigeminal ganglia (TG) and the dental pulp of the maxillary molar teeth from three male Sprague–Dawley rats weighing 300–330 g and dental pulps of three healthy human (male) maxillary premolar teeth from three 16 to 28‐year‐old patients extracted for orthodontic treatment were used. The type of VGLUT + axons that coexpress substance P (SP)‐ and/or calcitonin gene‐related peptide (CGRP) and parvalbumin in the rat TG and in the axons of the rat and the human dental pulp was examined by double fluorescence immunohistochemistry and quantitative analysis. Results were analyzed using one‐way anova and the Kruskal–Wallis test.
Results
SP and CGRP were expressed in many human VGLUT1 + pulpal axons but not in the rat VGLUT1 + TG neurons and pulpal axons (P < 0.05). SP and CGRP were expressed in a considerable number of human VGLUT2 + pulpal axons and also in many rat TG neurons and pulpal axons. The fraction of VGLUT1 + axons expressing parvalbumin was about three times higher in the rat than in the human dental pulp (P < 0.05).
Conclusions
These findings suggest that the types of VGLUT + axons, which release neuropeptides, may be different between the rat and the human dental pulp, raising a possibility that peripheral mechanism of pulpal inflammatory pain may be different between rats and humans.</description><subject>Animals</subject><subject>Axons</subject><subject>Calcitonin</subject><subject>Calcitonin gene-related peptide</subject><subject>Dental Pulp</subject><subject>Dentistry</subject><subject>Ganglia</subject><subject>glutamate</subject><subject>Glutamic acid transporter</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Inflammation</subject><subject>inflammatory pain</subject><subject>Kruskal-Wallis test</subject><subject>Maxilla</subject><subject>neuropeptide</subject><subject>Neuropeptides</subject><subject>Orthodontics</subject><subject>Pain</subject><subject>Parvalbumin</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Substance P</subject><subject>Teeth</subject><subject>Trigeminal ganglion</subject><subject>Vesicular Glutamate Transport Proteins</subject><subject>vesicular glutamate transporter</subject><issn>0143-2885</issn><issn>1365-2591</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp10c9qFTEUBvAgir1WF76ABNzYxbTJnMlMspTS1kqhm9LtkCZnbC4zyZg_td31EXxGn8TorS4EswkkPz4O5yPkLWeHvJ4jh9tDDl07PCMbDr1oWqH4c7JhvIOmlVLskVcpbRljggF_SfYAmGKdhA3J15icKbOO9Mtcsl50Rpqj9mkNMWP88fjdLUvxYQ3JZXeHVN8Hn2i-1ZmagPdrxJSoxxLDimt2FhN1vv4jjZVob-ltWbSnFn3WM13LvL4mLyY9J3zzdO-Tq9OTq-NPzcXl2fnxx4vGgIChGYZJTrZjPTDDrTTKTGigN8oOrdK219MNgBKdUlqpqpRp6xuIXjKcrIB98mEXu8bwtWDK4-KSwXnWHkNJY9sJ6KUSQlb6_h-6DSX6OlxVUvKuBwlVHeyUiSGliNO4Rrfo-DByNv5qYqxNjL-bqPbdU2K5WdD-lX9WX8HRDnxzMz78P2k8P_m8i_wJiCeVfA</recordid><startdate>202103</startdate><enddate>202103</enddate><creator>Cho, Y. S.</creator><creator>Ko, H. G.</creator><creator>Han, H. M.</creator><creator>Park, S. K.</creator><creator>Moozhayil, S. J.</creator><creator>Choi, S. Y.</creator><creator>Bae, Y. C.</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-7618-418X</orcidid></search><sort><creationdate>202103</creationdate><title>Vesicular glutamate transporter‐immunopositive axons that coexpress neuropeptides in the rat and human dental pulp</title><author>Cho, Y. S. ; Ko, H. G. ; Han, H. M. ; Park, S. K. ; Moozhayil, S. J. ; Choi, S. Y. ; Bae, Y. C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3537-77f8fd40630c1d8c9cfec36c9d729ad6afb3395499a990639c26af35680efd53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Axons</topic><topic>Calcitonin</topic><topic>Calcitonin gene-related peptide</topic><topic>Dental Pulp</topic><topic>Dentistry</topic><topic>Ganglia</topic><topic>glutamate</topic><topic>Glutamic acid transporter</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Inflammation</topic><topic>inflammatory pain</topic><topic>Kruskal-Wallis test</topic><topic>Maxilla</topic><topic>neuropeptide</topic><topic>Neuropeptides</topic><topic>Orthodontics</topic><topic>Pain</topic><topic>Parvalbumin</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Substance P</topic><topic>Teeth</topic><topic>Trigeminal ganglion</topic><topic>Vesicular Glutamate Transport Proteins</topic><topic>vesicular glutamate transporter</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cho, Y. S.</creatorcontrib><creatorcontrib>Ko, H. G.</creatorcontrib><creatorcontrib>Han, H. M.</creatorcontrib><creatorcontrib>Park, S. K.</creatorcontrib><creatorcontrib>Moozhayil, S. J.</creatorcontrib><creatorcontrib>Choi, S. Y.</creatorcontrib><creatorcontrib>Bae, Y. C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>International endodontic journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cho, Y. S.</au><au>Ko, H. G.</au><au>Han, H. M.</au><au>Park, S. K.</au><au>Moozhayil, S. J.</au><au>Choi, S. Y.</au><au>Bae, Y. C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Vesicular glutamate transporter‐immunopositive axons that coexpress neuropeptides in the rat and human dental pulp</atitle><jtitle>International endodontic journal</jtitle><addtitle>Int Endod J</addtitle><date>2021-03</date><risdate>2021</risdate><volume>54</volume><issue>3</issue><spage>377</spage><epage>387</epage><pages>377-387</pages><issn>0143-2885</issn><eissn>1365-2591</eissn><abstract>Aim
To examine the type of vesicular glutamate transporter (VGLUT)‐immunopositive (+) axons that coexpress neuropeptides in the rat and human dental pulp, which may help understand peripheral mechanism of pulpal inflammatory pain in rats and humans.
Methodology
The trigeminal ganglia (TG) and the dental pulp of the maxillary molar teeth from three male Sprague–Dawley rats weighing 300–330 g and dental pulps of three healthy human (male) maxillary premolar teeth from three 16 to 28‐year‐old patients extracted for orthodontic treatment were used. The type of VGLUT + axons that coexpress substance P (SP)‐ and/or calcitonin gene‐related peptide (CGRP) and parvalbumin in the rat TG and in the axons of the rat and the human dental pulp was examined by double fluorescence immunohistochemistry and quantitative analysis. Results were analyzed using one‐way anova and the Kruskal–Wallis test.
Results
SP and CGRP were expressed in many human VGLUT1 + pulpal axons but not in the rat VGLUT1 + TG neurons and pulpal axons (P < 0.05). SP and CGRP were expressed in a considerable number of human VGLUT2 + pulpal axons and also in many rat TG neurons and pulpal axons. The fraction of VGLUT1 + axons expressing parvalbumin was about three times higher in the rat than in the human dental pulp (P < 0.05).
Conclusions
These findings suggest that the types of VGLUT + axons, which release neuropeptides, may be different between the rat and the human dental pulp, raising a possibility that peripheral mechanism of pulpal inflammatory pain may be different between rats and humans.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>33090483</pmid><doi>10.1111/iej.13427</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-7618-418X</orcidid></addata></record> |
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| ispartof | International endodontic journal, 2021-03, Vol.54 (3), p.377-387 |
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| language | eng |
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| subjects | Animals Axons Calcitonin Calcitonin gene-related peptide Dental Pulp Dentistry Ganglia glutamate Glutamic acid transporter Humans Immunohistochemistry Inflammation inflammatory pain Kruskal-Wallis test Maxilla neuropeptide Neuropeptides Orthodontics Pain Parvalbumin Rats Rats, Sprague-Dawley Substance P Teeth Trigeminal ganglion Vesicular Glutamate Transport Proteins vesicular glutamate transporter |
| title | Vesicular glutamate transporter‐immunopositive axons that coexpress neuropeptides in the rat and human dental pulp |
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