Use of Alcalase in the production of bioactive peptides: A review

This review aims to cover the uses of the commercially available protease Alcalase in the production of biologically active peptides since 2010. Immobilization of Alcalase has also been reviewed, as immobilization of the enzyme may improve the final reaction design enabling the use of more drastic c...

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Bibliographic Details
Published in:International journal of biological macromolecules 2020-12, Vol.165 (Pt B), p.2143-2196
Main Authors: Tacias-Pascacio, Veymar G., Morellon-Sterling, Roberto, Siar, El-Hocine, Tavano, Olga, Berenguer-Murcia, Ángel, Fernandez-Lafuente, Roberto
Format: Article
Language:English
Subjects:
Bioactive peptides
Biocompatible Materials - metabolism
Combienzymes
Enzyme selectivity
Enzyme specificity
Enzymes, Immobilized - metabolism
Hydrolysis
Peptides - metabolism
Protease immobilization
Protein hydrolysis
Substrate Specificity
Subtilisins - metabolism
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Summary:This review aims to cover the uses of the commercially available protease Alcalase in the production of biologically active peptides since 2010. Immobilization of Alcalase has also been reviewed, as immobilization of the enzyme may improve the final reaction design enabling the use of more drastic conditions and the reuse of the biocatalyst. That way, this review presents the production, via Alcalase hydrolysis of different proteins, of peptides with antioxidant, angiotensin I–converting enzyme inhibitory, metal binding, antidiabetic, anti-inflammatory and antimicrobial activities (among other bioactivities) and peptides that improve the functional, sensory and nutritional properties of foods. Alcalase has proved to be among the most efficient proteases for this goal, using different protein sources, being especially interesting the use of the protein residues from food industry as feedstock, as this also solves nature pollution problems. Very interestingly, the bioactivities of the protein hydrolysates further improved when Alcalase is used in a combined way with other proteases both in a sequential way or in a simultaneous hydrolysis (something that could be related to the concept of combi-enzymes), as the combination of proteases with different selectivities and specificities enable the production of a larger amount of peptides and of a smaller size.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2020.10.060