Co‐culture model reveals the characteristics of theca cells and the effect of granulosa cells on theca cells at different stages of follicular development

Theca cells (TCs) play an important role in follicular development, which cannot be separated from granulosa cells (GCs). However, compared with mammals, the TCs and the effects of GCs on TCs at different follicular development stages (FDSs) have specific characteristics in avian species, but none o...

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Bibliographic Details
Published in:Reproduction in domestic animals 2021-01, Vol.56 (1), p.58-73
Main Authors: Gan, Xiang, Wang, Yushi, Gao, Shanyan, Chen, Xi, Hu, Shenqiang, Wang, Jiwen, Hu, Jiwei, Li, Liang, Han, Chunchun
Format: Article
Language:English
Subjects:
Apoptosis
Aquatic birds
Aromatase
Cell culture
Cell morphology
co‐culture
Cytology
follicular development stage
Gene expression
goose
granulosa cell
Granulosa cells
Lipids
Lipogenesis
Morphology
Steroidogenesis
Sterol regulatory element-binding protein
Theca
theca cell
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Summary:Theca cells (TCs) play an important role in follicular development, which cannot be separated from granulosa cells (GCs). However, compared with mammals, the TCs and the effects of GCs on TCs at different follicular development stages (FDSs) have specific characteristics in avian species, but none of them have been clearly defined. In this study, we established an in vitro co‐culture (with GC at the corresponding stage) model of goose TCs at different FDSs (pre‐hierarchical, hierarchical and F1) by using a transwell system. The properties of TCs in co‐culture at the three FDSs, including cell morphology, activity and intracellular lipid content, as well as the expression of key genes involved in de novo lipogenesis, steroidogenesis, proliferation and apoptosis, were examined and defined. We further compared the mono‐culture and co‐culture groups. After co‐culture, the activity of TCs showed significant (p 
ISSN:0936-6768
1439-0531
DOI:10.1111/rda.13849