Seed-specific activity of the Arabidopsis β-glucosidase 19 promoter in transgenic Arabidopsis and tobacco

• Published in: Plant cell reports 2021, Vol.40 (1), p.213-221
• Main Authors: Liu, Shijuan, Liu, Changju, Wang, Xue, Chen, Huiqing
• Format: Article
• Language: English
• Subjects: Arabidopsis; Arabidopsis - genetics; Arabidopsis Proteins - genetics; beta-Glucosidase - genetics; Bioinformatics; Biomedical and Life Sciences; Biotechnology; Cell Biology; Cellobiase; Cellulases - genetics; Cloning, Molecular; Gene expression; Gene Expression Regulation, Plant; Glucosidase; Glucuronidase - genetics; Life Sciences; Nicotiana - genetics; Organs; Original Article; Plant Biochemistry; Plant Sciences; Plants, Genetically Modified - genetics; Promoter Regions, Genetic; Regulatory Sequences, Nucleic Acid; Reporter gene; Seeds; Seeds - genetics; Tobacco; Transgenic plants; β-Glucosidase
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/s00299-020-02627-8

Key message The promoter of the Arabidopsis thaliana β-glucosidase 19 gene directs GUS expression in a seed-specific manner in transgenic Arabidopsis and tobacco. In the present study, an 898-bp putative promoter of the  Arabidopsis β-glucosidase 19 ( AtBGLU19 ) gene was cloned. The bioinformatics analysis of the cis -acting elements indicated that this putative promoter contains many seed-specific elements, such as RY elements. The features of this promoter fragment were evaluated for the capacity to direct the β-glucuronidase ( GUS ) reporter gene in transgenic Arabidopsis and tobacco. Histochemical and fluorometric GUS analyses of transgenic Arabidopsis plants revealed that the AtBGLU19 promoter directed strong GUS activity in late-maturing seeds and dry seeds, whereas no GUS expression was observed in other organs. The results indicated that the AtBGLU19 promoter was able to direct GUS expression in a seed-specific manner in transgenic Arabidopsis . In tobacco, the intensity of the staining and the level of GUS activity were considerably higher in the seeds than in the other tissues. These results further confirmed that the AtBGLU19 promoter is seed specific and can be used to control transgene expression in a heterologous plant system.