Current scenario of RNAi‐based hemipteran control

RNA interference (RNAi) is an homology‐dependent gene silencing mechanism that is a feasible and sustainable avenue for the management of hemipteran pests. Commercial implementation of RNAi‐based control strategies is impeded by limited knowledge about the mechanism of double‐stranded RNA (dsRNA) up...

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Bibliographic Details
Published in:Pest management science 2021-05, Vol.77 (5), p.2188-2196
Main Authors: Jain, Ritesh G, Robinson, Karl E, Asgari, Sassan, Mitter, Neena
Format: Article
Language:English
Subjects:
Animals
Biodegradation
Double-stranded RNA
dsRNA
Gene expression
Gene Silencing
Hemiptera
hemipteran pest control
Homology
Insect Control
Insecta - genetics
Insecticide resistance
Insecticides
Insects
non‐transformative RNAi
Pest Control
Pesticide resistance
Pests
Ribonucleic acid
RNA
RNA Interference
RNA, Double-Stranded - genetics
RNA-mediated interference
RNAi
Toxicity
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Summary:RNA interference (RNAi) is an homology‐dependent gene silencing mechanism that is a feasible and sustainable avenue for the management of hemipteran pests. Commercial implementation of RNAi‐based control strategies is impeded by limited knowledge about the mechanism of double‐stranded RNA (dsRNA) uptake, the function of core RNAi genes and systemic RNAi mechanisms in hemipteran insects. This review briefly summarizes recent progress in RNAi‐based studies aimed to reduce insect populations, viral transmission and insecticide resistance focusing on hemipteran pests. This review explores RNAi‐mediated management of hemipteran insects and offers potential solutions, including in silico approaches coupled with laboratory‐based toxicity assays to circumvent potential off‐target effects against beneficial organisms. We further explore ways to mitigate degradation of dsRNA in the environment and the insect such as stacking and formulation of dsRNA effectors. Finally, we conclude by considering nontransformative RNAi approaches, concatomerization of RNAi sequences and pyramiding RNAi with active constituents to reduce dsRNA production and application cost, and to improve broad‐spectrum hemipteran pest control. © 2020 Society of Chemical Industry This review highlights the recent advances in RNAi‐mediated management of hemipteran insects and provides future research strategies for improving the potential RNAi‐based biopesticides.
ISSN:1526-498X
1526-4998
DOI:10.1002/ps.6153