Effects of photobiomodulation and swimming on gene expression in rats with the tibialis anterior muscle injury

• Published in: Lasers in medical science 2021-09, Vol.36 (7), p.1379-1387
• Main Authors: Beasi, W. R., Toffoli, L. V., Pelosi, G. G., Gomes, M. V. M., Verissimo, L. F., Stocco, M. R., Mantoani, L. C., Maia, L. P., Andraus, R. A. C.
• Format: Article
• Language: English
• Subjects: Aluminum gallium arsenide lasers; Cryoinjury; Dentistry; Flux density; Gene expression; Genes; Injuries; Lasers; Light therapy; Medicine; Medicine & Public Health; Muscles; MyoD protein; Myogenin; Optical Devices; Optics; Original Article; Photonics; Physical training; Quantum Optics; Regeneration; Skeletal muscle; Swimming; Tibialis anterior muscle; Transforming growth factor-b
• ISSN: 0268-8921; 1435-604X
• DOI: 10.1007/s10103-020-03168-y

The aim of the present study was to evaluate the effects of photobiomodulation (low-level laser therapy (LLLT)) and aquatic exercise on the expression of genes related to muscle regeneration in rats. Wistar rats were divided into five groups: control group ( n = 15), non-treated injury group ( n = 15), injury+LLLT group ( n = 15), injury+aquatic exercise group ( n = 15), and injury+LLLT+aquatic exercise group ( n = 15). Cryoinjury was performed on the belly of the tibialis anterior (TA) muscle. LLLT was performed daily with an AlGaAs laser (830 nm; beam spot of 0.0324 cm 2 , output power of 100 mW, energy density of 180 J/cm 2 , and 58-s exposure time). Animals were euthanized at 7, 14, and 21 days. The TA muscles were removed for gene expression analysis of TGF-β, Myogenin, and MyoD. The results were statistically analyzed at a significance level of 5%. The cryoinjury increased the expression of genes related to muscle regeneration—MyoD, Myogenin, and TGF-β—compared to the control group ( p < 0.05); the photobiomodulation increased the expression of these genes at day 7 ( p < 0.05), decreasing until day 21; and the aquatic exercise increases the expression of the three genes over time. When the two treatments were combined, the expression of the analyzed genes also increased over time. In summary, the results of our study suggest that photobiomodulation (LLLT), when applied alone in cryoinjury, is able to increase the gene expression of MyoD, Myogenin, and TGF-β at the acute phase, while when combined with aquatic exercises, there is an increase in expression of these genes specially at the long-term treatment.