Down-regulation of lncRNA PCGEM1 inhibits cervical carcinoma by modulating the miR-642a-5p/LGMN axis

• Published in: Experimental and molecular pathology 2020-12, Vol.117, p.104561-104561, Article 104561
• Main Authors: Liu, Yuanlin, Wang, Ye, Shen, Xiang, Chen, Chen, Ni, Huihua, Sheng, Nan, Hua, Minhui, Wu, Yanling
• Format: Article
• Language: English
• Subjects: Carcinoma - genetics; Carcinoma - pathology; Cell Line, Tumor; Cell Movement - genetics; Cell Proliferation - genetics; Cervical carcinoma; Cysteine Endopeptidases - genetics; Female; Gene Expression Regulation, Neoplastic - genetics; Humans; LGMN; MicroRNAs - genetics; Migration; miR-642a-5p; Neoplasm Invasiveness - genetics; Neoplasm Invasiveness - pathology; PCGEM1; Proliferation; RNA, Long Noncoding - genetics; Signal Transduction - genetics; Uterine Cervical Neoplasms - genetics; Uterine Cervical Neoplasms - pathology
• ISSN: 0014-4800; 1096-0945
• DOI: 10.1016/j.yexmp.2020.104561

LncRNA PCGEM1 (PCGEM1) has been reported to exert essential effects on the development and progress of various tumors, while the detailed effects and possible mechanisms of PCGEM1 in cervical carcinoma remain unknown. In the present study, PCGEM1 was over-expressed in cervical carcinoma cells as evidenced by real-time quantitative polymerase chain reaction (RT-qPCR) assay. Knockdown of PCGEM1 significantly repressed proliferation, migration, and invasion, while induced G1 arrest in cervical carcinoma cells. In addition, PCGEM1 was predicted to target miR-642a-5p by bioinformatics software, which was further confirmed by luciferase reporter assay. Besides, RT-qPCR assay indicated that miR-642a-5p expression was decreased in cervical carcinoma cells and knockdown of PCGEM1 could accelerate miR-642a-5p expression. Moreover, inhibition of miR-642a-5p partly abolished the functions of PCGEM1 knockdown on proliferation, cell cycle, migration and invasion of cervical carcinoma cells. Furthermore, miR-642a-5p could bind to the 3’-UTR of LGMN, which was over-expressed in the cervical carcinoma cells. Suppression of LGMN partly restored the functions of miR-642a-5p inhibitor on proliferation, cell cycle distribution, migration and invasion in the cervical carcinoma cells treated with the PCGEM1 shRNA. Taken together, our data indicated that knockdown of PCGEM1 inhibited proliferation, migration and invasion in cervical carcinoma by modulating the miR-642a-5p/ LGMN axis.