Uncovering the expression patterns and the clinical significance of miR-182, miR-205, miR-27a and miR-369 in patients with urinary bladder cancer

• Published in: Molecular biology reports 2020-11, Vol.47 (11), p.8819-8830
• Main Authors: Setti Boubaker, Nouha, Gurtner, Aymone, Trabelsi, Nesrine, Manni, Isabella, Ayed, Haroun, Saadi, Ahmed, Naimi, Zeineb, Ksontini, Meriem, Ayadi, Mouna, Blel, Ahlem, Rammeh, Soumaya, Chebil, Mohamed, Piaggio, Giulia, Ouerhani, Slah
• Format: Article
• Language: English
• Subjects: Animal Anatomy; Animal Biochemistry; Biomarkers; Biomedical and Life Sciences; Bladder cancer; Clinical significance; Histology; Life Sciences; miRNA; Morphology; Original Article; Patients; Prognosis; Statistical analysis; Tumors; Urinary bladder; Urothelial cancer
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-020-05932-3

Background Given the high recurrence and progression rates and the absence of reliable markers for early detection and prognosis prediction of patients with urothelial bladder cancer (BCa), the exploration of new biomarkers with high specificity is imperative. Mainly, microRNAs (miRNAs), which are involved in the initiation and the progression of BCa. Herein, the expression patterns of miR-182, miR-205, miR-27a and miR-369 were evaluated in patients with urothelial BCa. Methods and results The expression levels of the miRNAs were investigated in 90 FFPE tissue samples (23 LG NMIBC, 44 HG NMIBC, 23 MIBC) and 10 non tumoral bladder tissues using TaqMan based RT-qPCR. Data analysis was performed using 2 −ΔΔCT method. Correlation to clinical characteristics of the patients was performed using descriptive statistics and the receiver operating characteristic (ROC) curve was performed to evaluate the diagnostic value of all miRNAs. MiR-27a, miR-205 and miR-369 were down-regulated whereas miR-182 was up-regulated in patients compared to controls ( p