A Higher Level of Expression of the Nucleolar Protein SURF6 in Human Normal Activated Lymphocytes and in Lymphocytes of Patients with Lymphoproliferative Disorders

Proliferation of mammalian cells is often accompanied by an increase in the content of the nucleolar proteins, which allows researchers to consider such proteins as potential activation markers. To test this assumption experimentally, we examined the expression pattern of the nucleolar rRNA processi...

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Bibliographic Details
Published in:Doklady. Biochemistry and biophysics 2020-09, Vol.494 (1), p.261-265
Main Authors: Moraleva, A. A., Malysheva, M. A., Khajdukov, S. V., Zatsepina, O. V.
Format: Article
Language:English
Subjects:
Biochemistry
Biological and Medical Physics
Biomarkers - metabolism
Biomedical and Life Sciences
Biophysics
Case-Control Studies
Cell activation
Cell Nucleolus - immunology
Cell proliferation
Humans
Immunoblotting
Immunofluorescence
Immunoproliferative diseases
Life Sciences
Lymphatic diseases
Lymphatic leukemia
Lymphocyte Activation
Lymphocytes
Lymphocytes - cytology
Lymphocytes - immunology
Lymphoproliferative Disorders - metabolism
Lymphoproliferative Disorders - pathology
Mammalian cells
Molecular Biology
Nuclear Proteins - biosynthesis
Nuclear Proteins - immunology
Nucleoli
Peripheral blood
Proteins
RNA processing
RNA, Ribosomal - metabolism
rRNA
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Summary:Proliferation of mammalian cells is often accompanied by an increase in the content of the nucleolar proteins, which allows researchers to consider such proteins as potential activation markers. To test this assumption experimentally, we examined the expression pattern of the nucleolar rRNA processing factor SURF6 in normal (resting) peripheral blood lymphocytes, lymphocytes activated for proliferation in vitro, and in blood samples from patients with lymphoproliferative diseases. Using two methods (immunofluorescence and immunoblotting), we for the first time showed that the SURF6 protein is not detected in normal lymphocytes but can easily be visualized in lymphocytes after PHA activation and in lymphocytes of lymphocytic leukemia patients. The level of SURF6 expression in patients correlated with the aggressiveness of the disease development determined by the content of Ki-67-positive lymphocytes. These results allow the SURF6 nucleolar protein to be considered as a putative activation marker of lymphocytes in human blood disorders.
ISSN:1607-6729
1608-3091
DOI:10.1134/S1607672920050099