Stem lodging resistance in hulless barley: Transcriptome and metabolome analysis of lignin biosynthesis pathways in contrasting genotypes

Hulless barley is an important economic and food crop for local population in the Qinghai-Tibet plateau. However, due to extreme weather conditions, its production suffers from stem lodging stress, inflicting significant yield losses. Herein, we selected five lodging resistant and five non-resistant...

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Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 2021-01, Vol.113 (1), p.935-943
Main Authors: Yu, Mingzhai, Wang, Mu, Gyalpo, Thondup, Basang, Yuzhen
Format: Article
Language:English
Subjects:
Crop Production - methods
Gene expression
Genotype
Hordeum - genetics
Hordeum - growth & development
Hordeum - metabolism
Hulless barley
Lignin - biosynthesis
Lignin - genetics
Lignin formation
Lodging resistance
Metabolome
Plant Stems - genetics
Plant Stems - growth & development
Quantitative Trait, Heritable
Transcriptome
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Summary:Hulless barley is an important economic and food crop for local population in the Qinghai-Tibet plateau. However, due to extreme weather conditions, its production suffers from stem lodging stress, inflicting significant yield losses. Herein, we selected five lodging resistant and five non-resistant genotypes to investigate changes in concentration of lignin related metabolites and expression levels of related genes in node samples. The lodging resistant genotypes displayed high content of lignin intermediate metabolites. 57% of the expressed genes were differentially expressed (DEG) between the two groups. 31 DEGs participate in the lignin pathways and we found that 65% of these DEGs were strongly up-regulated in the lodging resistant group, indicating a mechanism towards high lignin synthesis within said group. The candidate structural genes as well as the co-expressed TFs identified in this study represent important molecular tools for functional characterization and exploitation in molecular breeding programmes. •Lodging resistant hulless barley genotypes had high content of lignin related metabolites.•Transcriptome of 10 contrasting genotypes was analysed for lignin related genes.•Most of lignin related genes were higher expressed in the lodging resistant group.•Transcription factors co-expressed with lignin related genes were identified.
ISSN:0888-7543
1089-8646
DOI:10.1016/j.ygeno.2020.10.027