Halomonas bachuensis sp. nov., Isolated from Gobi Soil

• Published in: Current microbiology 2021, Vol.78 (1), p.397-402
• Main Authors: Xiao, Zijun, Shen, Jie, Wang, Zhuo, Dong, Fanghui, Zhao, Jing-yi
• Format: Article
• Language: English
• Subjects: Bacterial Typing Techniques; Base Composition; Biomedical and Life Sciences; Biotechnology; Cardiolipin; China; Deoxyribonucleic acid; DNA; DNA, Bacterial - genetics; Endophytes; Fatty Acids; Gene sequencing; Gram stain; Halomonas; Halomonas - genetics; Hybridization; Life Sciences; Lipids; Microbiology; New species; Nucleotides; pH effects; Phosphatidylethanolamine; Phosphatidylglycerol; Phospholipids; Phylogenetics; Phylogeny; Quinones; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, DNA; Sodium chloride; Soil; Soil bacteria; Soil microorganisms; Ubiquinone
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-020-02268-w

A novel aerobic bacterium designated DX6 T was isolated from a Gobi soil sample collected in Bachu County, China. Cells are Gram-stain-negative and rod-shaped and colonies are creamy, circular and smooth. The growth range of NaCl concentration was 1–15% (optimum 2–10%, w / v ). Growth occurs at 10–45 °C (optimum 37 °C) and pH 5.0–10.0 (optimum pH 7.0–9.0). Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain DX6 T formed a distinct lineage in the clade of genus Halomonas and is related to Halomonas desiderata DSM 9502 T (98.3%), Halomonas kenyensis AIR-2 T (97.7%), Halomonas daqingensis DQD2-30 T (97.6%), Halomonas saliphila LCB169 T (97.4%) and Halomonas endophytica MC28 T (96.2%). Analysis of the housekeeping genes gryB and rpoD and calculation of the average nucleotide identities and the digital DNA-DNA hybridization values between strain DX6 T and the related type Halomonas strains further revealed that strain DX6 T represented a distinct species. The main respiratory quinones of strain DX6 T were ubiquinone 9 (Q-9) and ubiquinone 8 (Q-8). The predominant cellular fatty acids were summed feature 8 (C 18:1 ω 7c and/or C 18:1 ω 6c), summed feature 3 (C 16:1 ω 7c and/or C 16:1 ω 6c) and C 16:0 . The major polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, an unidentified phosphatidylglycolipid, and four unidentified lipids. Based on the phenotypic, phylogenetic, chemotaxonomic and genomic features, strain DX6 T represents a novel species of the genus Halomonas . The name Halomonas bachuensis sp. nov. is proposed with strain DX6 T (= CCTCC AB 2020094 T  = KCTC 82196 T ) designated as the type strain.