Elucidating the processes and pathways enriched in buffalo sperm proteome in regulating semen quality

Sperm carries a reservoir of proteins regulating the molecular functions to attain functional competence. Semen samples collected from buffalo bulls were assessed for sperm functional attributes ( n  = 11) and proteome profiling ( n  = 6). Sperm proteins were extracted and profiled by employing LC–M...

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Bibliographic Details
Published in:Cell and tissue research 2021-02, Vol.383 (2), p.881-903
Main Authors: Binsila, Bala Krishnan, Archana, Santhanahalli Siddalingappa, Ramya, Laxman, Swathi, Divakar, Selvaraju, Sellappan, Gowda, NK. Shivakumar, Pal, Din Taran, Rafay, Abu, Bhatta, Raghavendra
Format: Article
Language:English
Subjects:
Acrosin
Biomedical and Life Sciences
Biomedicine
Embryogenesis
Embryonic development
Fertilization
Fluid flow
Human Genetics
Isoleucine
Leucine
Mechanical stimuli
Meiosis
Mesenchyme
Metabolic pathways
Molecular Medicine
Morphogenesis
Muscle contraction
Phosphatidylethanolamine
Proteinase inhibitors
Proteins
Proteomes
Proteomics
Regular Article
Semen
Serine
Serine proteinase
Sexual reproduction
Smooth muscle
Sperm
Spermatozoa
Thermogenesis
Valine
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Summary:Sperm carries a reservoir of proteins regulating the molecular functions to attain functional competence. Semen samples collected from buffalo bulls were assessed for sperm functional attributes ( n  = 11) and proteome profiling ( n  = 6). Sperm proteins were extracted and profiled by employing LC–MS/MS. Overall, the buffalo sperm contained 1365 proteins, of which 458 were common between the groups. The unique proteins were 477 and 430 in good and poor quality semen, respectively. In the whole proteome of buffalo sperm, sexual reproduction with phosphatidylethanolamine-binding protein1 (PEBP1), fetuin-B (FETUB) and acrosin (ACR) was the most enriched ( p  = 8.44E−19) biological process, also with thermogenesis ( p  = 0.003), oocyte meiosis ( p  = 0.007) and vascular smooth muscle contraction ( p  = 0.009) apart from metabolic pathways. In good quality semen, mesenchyme migration ( p  = 1.24E−07) and morphogenesis ( p  = 0.001) were abundant biological processes. In good quality semen, the fluid shear stress ( p  = 0.01) and, in poor quality semen, valine, leucine and isoleucine degradation ( p  = 3.8E−05) pathways were enriched. In good quality semen, 7 proteins were significantly ( p  
ISSN:0302-766X
1432-0878
DOI:10.1007/s00441-020-03303-9