Broadly Tuned Odorant Receptor AlinOR59 Involved in Chemoreception of Floral Scent in Adelphocoris lineolatus

• Published in: Journal of agricultural and food chemistry 2020-11, Vol.68 (47), p.13815-13823
• Main Authors: Xiao, Yong, An, Xing-Kui, Khashaveh, Adel, Shan, Shuang, Wang, Qi, Wang, Shan-Ning, Gu, Shao-Hua, Li, Zhen-Yu, Zhang, Yong-Jun
• Format: Article
• Language: English
• Subjects: Animals; Arthropod Antennae; Chemistry and Biology of Aroma and Taste; Female; Flowers - chemistry; Heteroptera; Insect Proteins - genetics; Male; Odorants; Receptors, Odorant - genetics; Sensilla
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/acs.jafc.0c04434

Plant volatiles such as floral scent compounds play a crucial role in mediating insect host locating, mate search, and oviposition sites selection. The alfalfa plant bug, Adelphocoris lineolatus (Goeze), is a seriously polyphagous herbivore of alfalfa and cotton that has an obvious preference for flowering host plants. In this study, we focused on the role of an odorant receptor AlinOR59 in the perception of plant volatiles in A. lineolatus. In situ hybridization showed that AlinOR59 was coexpressed with the coreceptor AlinORco in the ORNs cell located in the long curved sensilla trichodea on antennae of both genders. The Xenopus oocytes expression coupled with two-electrode voltage clamp recordings demonstrated that AlinOR59 responded to 15 plant volatiles. In electroantennogram assays, all of the above 15 compounds could excite electrophysiological responses in the antennae of adult bugs. Furthermore, an important floral scent compound, methyl salicylate, was utilized to evaluate the behavioral responses of A. lineolatus. It was found that adult bugs of both genders were significantly attracted to methyl salicylate. Taken together, our findings suggest that AlinOR59 plays a crucial role in the perception of floral scents in A. lineolatus and could be used as a potential target to design novel olfactory regulators for the management of bugs.