Determination of bioactive compounds of Artemisia Spp. plant extracts by LC–MS/MS technique and their in-vitro anti-adipogenic activity screening

• Published in: Journal of pharmaceutical and biomedical analysis 2021-01, Vol.193, p.113707-113707, Article 113707
• Main Authors: Singh, Pratibha, Bajpai, Vikas, Khandelwal, Nilesh, Varshney, Salil, Gaikwad, Anil N., Srivastava, Mukesh, Singh, Bikarma, Kumar, Brijesh
• Format: Article
• Language: English
• Subjects: Artemisia species; In-vitro anti-adipogenic activity; Multiple reaction monitoring (MRM) mode; UPLC-ESI-QqQLIT-MS/MS; Validation
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/j.jpba.2020.113707

[Display omitted] •UPLC-ESI-QqQLIT-MS/MS method was developed and validated in polarity switching MRM mode and successfully applied in ethanolic extract of Artemisia species for the quantitation of twelve bioactive compounds.•Dihydroartemisinin, artemether and salicylic acid were abundant in A. dracunculus while rutin and artemisinin were highest in A. vestita and A. absinthium respectively.•The statistical analysis for comparison of observed quantitative differences of each compound was done to show that they are statistically significant.•In-vitro assessment of extracts of selected Artemisia species were screened for their potential in adipocyte differentiation in 3T3-L1 cells. Ultra Performance Liquid Chromatography coupled with hybrid triple quadrupole linear ion trap tandem mass spectrometry (UPLC-ESI-QqQLIT-MS/MS) method in multiple reaction monitoring (MRM) acquisition mode was developed and validated for identification and simultaneous determination of potential anti-diabetic and anti-malarial compounds in ethanolic extracts of different Artemisia species. The chromatographic separation was carried out on an Acquity BEH™ C18 column (1.7 μm, 2.1 × 50 mm) with 0.1 % (v/v) formic acid in water and acetonitrile as mobile phase under gradient condition in 6 min. The developed method was validated in terms of linearity, LOD, LOQ, precision, stability and recovery according to international conference on harmonization guidelines. The correlation coefficients of all the calibration curves were ≥0.9902 and recoveries ranged from 98.22 to 104.49% (RSD ≤2.18 %). Relative standard deviations of intra-day, inter-day precisions and stability were ≤ 1.04, 1.09 and 2.80 %, respectively. The quantitative results showed remarkable differences in the content of all the compounds in different Artemisia species. The quantitative values of each peak were summarized as mean ± SD. The statistical analysis for comparison of observed quantitative differences of each compound was done to show that they are statistically significant. In-vitro assessment of extracts of selected Artemisia species inhibited adipocyte differentiation in 3T3-L1 cells, hence it may have certain phytochemicals which are responsible for reducing obesity and related metabolic disorders.