Optimal frame rate when there were stallion sperm motility evaluations and determinations for kinematic variables using CASA-Mot analysis in different counting chambers

• Published in: Animal reproduction science 2020-12, Vol.223, p.106643-106643, Article 106643
• Main Authors: Gacem, Sabrina, Bompart, Daznia, Valverde, Anthony, Catalán, Jaime, Miró, Jordi, Soler, Carles
• Format: Article
• Language: English
• Subjects: Capillary loaded chambers; Chamber depth; Drop displacement chambers; Field; Frame rate
• ISSN: 0378-4320; 1873-2232
• DOI: 10.1016/j.anireprosci.2020.106643

•High resolution cameras and counting chambers can define much better the stallion sperm movement.•The stallion sperm motility parameters are affected by the frame rate and the chamber.•The stallion sperm has higher velocity in reusable chambers than disposable chambers.•The sperm velocity is also higher in 20 μm chambers than 10 μm chambers.•The capture field in different chambers affect sperm kinematic parameters. This study was conducted to determine optimum image capture frame rates (FRO) when there was evaluation of different types of counting chambers used for CASA-Mot determinations of stallion sperm motility. Sperm VCL was determined at frame rates of 25–250 f/s in: 1) Spermtrack® (Spk) 10 and 20 chambers (drop displacement-type chambers 10 and 20 μm-deep respectively; and 2) ISAS®D4C10, ISAS®D4C20 (10 and 20 μm-deep respectively) and ISAS®D4C20 L (20 μm-deep) capillary loaded chambers. Values for different sperm kinematic variables were determined using each chamber at 250 f/s, which is the maximum frame rate that the software can be used for analyses. With evaluation of Spk chambers, there was a greater curvilinear velocity (VCL), average path velocity (VAP), straight line velocity (STR), amplitude of lateral head displacement (ALH) and beat cross frequency (BCF) values (P