Synthesis of linear polyglucoside and inhibition on the amyloid fibril formation of hen egg white lysozyme

• Published in: International journal of biological macromolecules 2021-01, Vol.166, p.771-777
• Main Authors: Wang, Mengna, Wang, Sujuan, Li, Benye, Tian, Yuelan, Zhang, Haisong, Bai, Libin, Ba, Xinwu
• Format: Article
• Language: English
• Subjects: Amyloid - chemistry; Amyloid - metabolism; Amyloid fibril; Animals; Chickens; Dose-dependent; Egg Proteins - chemistry; Egg Proteins - metabolism; Glucosides - chemical synthesis; Glucosides - pharmacology; HEWL; Inhibition; Methacrylates - chemistry; Muramidase - chemistry; Muramidase - metabolism; Poly(6-O-MMAGlc); Protein Multimerization - drug effects
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2020.10.234

A novel polymer poly (6-O-MMAGlc) has been synthesized via free radical polymerization of monomer methyl 6-O-methacryloyl-α-D-glucoside (6-O-MMAGlc) and characterized. The influence of poly(6-O-MMAGlc) on the formation of hen egg white lysozyme (HEWL) amyloid fibril was detailly investigated, indicating that the polymer could effectively inhibit the formation of HEWL amyloid fibril. The formation kinetics of HEWL amyloid fibril with the presence of poly(6-O-MMAGlc) was measured by Thioflavin T (ThT) fluorescence method, demonstrating that poly(6-O-MMAGlc) could significantly inhibit the amyloid fibril formation of HEWL in a dose-dependent manner. The inhibitory result was furtherly illustrated by congo red (CR) binding assay, 8-anilino-1-naphthalenesulfonic acid (ANS) fluorescence assay, circular dichroism (CD) spectroscopy and transmission electron microscope (TEM). •A novel polyglucoside was synthesized.•The polyglucoside can inhibit the formation of HEWL amyloid fibrils.•The inhibitory effectivity was in a dose-dependent manner.