Determinants of plasma fibrin clot lysis measured using three different assays in healthy subjects

Several methods for measuring fibrinolytic capacity in plasma have been developed yielding frequently inconsistent results. We investigated which factors determine fibrinolytic capacity in three plasma-based assays. In 80 apparently healthy controls (aged 43 ± 10 years, 50 women [62.5%]) we evaluate...

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Published in:Thrombosis research 2021-01, Vol.197, p.1-7
Main Authors: Siudut, Jakub, Iwaniec, Teresa, Plens, Krzysztof, Pieters, Marlien, Undas, Anetta
Format: Article
Language:English
Subjects:
Clot lysis time
CRP
Fibrinolytic capacity
HRG
γ′-Fibrinogen
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description Several methods for measuring fibrinolytic capacity in plasma have been developed yielding frequently inconsistent results. We investigated which factors determine fibrinolytic capacity in three plasma-based assays. In 80 apparently healthy controls (aged 43 ± 10 years, 50 women [62.5%]) we evaluated fibrinolysis using three assays: (1) by Pieters et al. (CLT2018), (2) by Lisman et al. (CLT), and (3) by Carter et al. (Lys50). Coagulation factors and fibrinolytic proteins, including histidine-rich glycoprotein (HRG) and γ′-fibrinogen, were determined. Regression models were performed to identify determinants of lysis times. Positive correlations were observed between CLT2018 and both CLT (r = 0.73) and Lys50 (r = 0.61), as well as between CLT and Lys50 (r = 0.46, all p 
doi_str_mv 10.1016/j.thromres.2020.10.014
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Histidine-rich glycoprotein was a predictor of the longest-normal CLT2018 alone (OR 1.04, 95% CI 1.02–1.06). α2-Antiplasmin and fibrinogen levels, followed by PAI-1 and TAFI determined Lys50. After adjustment for age, sex, and body mass index, C-reactive protein (CRP) was an independent predictor of the top quartiles of the three lysis times. We showed that apart from PAI-1, TAFI, and α2-antiplasmin, several other factors, in particular CRP, can affect the results of global fibrinolysis tests used in research. 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Histidine-rich glycoprotein was a predictor of the longest-normal CLT2018 alone (OR 1.04, 95% CI 1.02–1.06). α2-Antiplasmin and fibrinogen levels, followed by PAI-1 and TAFI determined Lys50. After adjustment for age, sex, and body mass index, C-reactive protein (CRP) was an independent predictor of the top quartiles of the three lysis times. We showed that apart from PAI-1, TAFI, and α2-antiplasmin, several other factors, in particular CRP, can affect the results of global fibrinolysis tests used in research. 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subjects Clot lysis time
CRP
Fibrinolytic capacity
HRG
γ′-Fibrinogen
title Determinants of plasma fibrin clot lysis measured using three different assays in healthy subjects
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