Production of a full chimeric mouse x pig anti-porcine DEC205 receptor recombinant antibody

Recombinant hybrid antibodies are commonly used in antigen-targeting assays to reduce the immunogenic potential associated with using classic mouse antibodies in other species. The DEC205 receptor has become an attractive target due to its effectiveness in activating the immune response and is consi...

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Bibliographic Details
Published in:Journal of immunological methods 2021-02, Vol.489, p.112911-112911, Article 112911
Main Authors: Bustamante-Córdova, Lorena, Melgoza-González, Edgar Alonso, Parra-Sánchez, Héctor, Reséndiz-Sandoval, Mónica, Burgara-Estrella, Alexel Jesús, Hernández, Jesús
Format: Article
Language:English
Subjects:
Antibody production
Chimeric antibody
DEC205
Dendritic cell
Recombinant antibody
Swine
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Summary:Recombinant hybrid antibodies are commonly used in antigen-targeting assays to reduce the immunogenic potential associated with using classic mouse antibodies in other species. The DEC205 receptor has become an attractive target due to its effectiveness in activating the immune response and is considered a promising vaccination target. The aim of this study was to produce a fully chimeric mouse x pig anti-porcine DEC205 recombinant antibody (rAb). Based on a mouse anti-porcine DEC205 monoclonal antibody (mAb), we designed and expressed a chimeric mouse x pig rAb using the Expi293f system. The resulting rAb maintained the recognition capacity of the native mouse mAb toward the porcine DEC205 receptor, as evidenced by western blot analysis. By using flow cytometry, we evaluated the ability of the rAb to recognize DEC205+ dendritic cells. In conclusion, the chimeric mouse x pig anti-DEC205 rAb can be used in antigen-targeting assays as a vaccination strategy in pigs. [Display omitted] •A chimeric mouse x pig anti-porcine DEC205 receptor recombinant antibody was produced•The chimeric recombinant antibody showed a recognition capacity similar to that of the original mouse antibody•The chimeric recombinant antibody recognized DEC205+ DCs, indicating that it is suitable for antigen targeting in pigs
ISSN:0022-1759
1872-7905
DOI:10.1016/j.jim.2020.112911