Diagnostic potential of peri‐implant crevicular fluid microRNA‐21‐3p and microRNA‐150‐5p and extracellular vesicles in peri‐implant diseases

Background To explore the diagnostic usefulness of extracellular vesicles (EVs), and their subpopulations (micro‐vesicles and exosomes), and microRNAs (miRNA‐21‐3p, miRNA‐150‐5p, and miRNA‐26a‐5p) in peri‐implant crevicular fluid (PICF) of subjects with healthy, peri‐implant mucositis and peri‐impla...

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Published in:Journal of periodontology (1970) 2021-06, Vol.92 (6), p.11-21
Main Authors: Chaparro, Alejandra, Atria, Pablo, Realini, Ornella, Monteiro, Lara J., Betancur, Daniel, Acuña‐Gallardo, Stephanie, Ramírez, Valeria, Bendek, María José, Pascual, Andrés, Nart, José, Beltrán, Victor, Sanz, Antonio
Format: Article
Language:English
Subjects:
Dental Implants - adverse effects
Extracellular Vesicles
Gingival Crevicular Fluid
Humans
MicroRNAs
Peri-Implantitis - diagnosis
peri‐implant mucositis
peri‐implantitis
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Summary:Background To explore the diagnostic usefulness of extracellular vesicles (EVs), and their subpopulations (micro‐vesicles and exosomes), and microRNAs (miRNA‐21‐3p, miRNA‐150‐5p, and miRNA‐26a‐5p) in peri‐implant crevicular fluid (PICF) of subjects with healthy, peri‐implant mucositis and peri‐implantitis implants. Methods A total of 54 patients were enrolled into healthy, peri‐implant mucositis, and peri‐implantitis groups. PICF samples were collected, EVs subpopulations (MVs and Exo) were isolated and characterized by nanoparticle tracking analysis and transmission electron microscopy. The expression of miRNA‐21‐3p, miRNA‐150‐5p and miRNA‐26a‐5p was quantified by qRT‐PCR. Logistic regression models and accuracy performance tests were estimated. Results PICF samples show the presence of EVs delimited by a bi‐layered membrane, in accordance with the morphology and size (
ISSN:0022-3492
1943-3670
DOI:10.1002/JPER.20-0372