Characterization of an oxidized alginate-gelatin hydrogel incorporating a COS-salicylic acid conjugate for wound healing

• Published in: Carbohydrate polymers 2021-01, Vol.252, p.117145-117145, Article 117145
• Main Authors: Oh, Gun-Woo, Kim, Se-Chang, Kim, Tae-Hee, Jung, Won-Kyo
• Format: Article
• Language: English
• Subjects: Alginates - chemistry; Animals; Antioxidants - chemistry; Antioxidants - pharmacology; Biocompatible Materials - chemistry; Biocompatible Materials - pharmacology; Chitin - analogs & derivatives; Chitin - chemistry; Chitin - pharmacology; Chitooligosaccharide–salicylic acid conjugate; Gelatin; Gelatin - chemistry; HaCaT Cells; Humans; Hydrogels - chemistry; Male; Mice; Mice, Inbred ICR; Oxidized alginate; Salicylic Acid - chemistry; Salicylic Acid - pharmacology; Schiff’s base reaction; Wound healing; Wound Healing - drug effects
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2020.117145

[Display omitted] •This study was to conjugate the chitooligosaccharide (COS) with salicylic acid (SA).•SA was conjugated to COS backbone by using a free radical-induced grafting method.•The COS-SA conjugates were completely synthesized and improved antioxidant activity.•Hydrogel was to fabricate using oxidized alginate (OA) and gelatin (G) with COS-SA.•Hydrogel with COS-SA conjugate exhibited wound healing activity in a mouse model. In the present study, chitooligosaccharide (COS) and salicylic acid (SA) conjugates were synthesized using H2O2-induced grafting polymerization and oxidized alginate (OA) and gelatin hydrogels were fabricated using Schiff’s base reaction. To confirm the synthesis and improved activity of the COS-SA conjugates, polyphenol assays, ultraviolet absorbance, NMR, and antioxidant activity tests were all conducted. The results showed that the COS-SA conjugates were completely synthesized and improved antioxidant activity. OA was synthesized using a periodate method and a hydrogel was obtained at a mixed ratio of 3:7 via crosslinking between the aldehyde group of the OA and the amine groups of gelatin with COS-SA conjugates. In a cytotoxicity and wound healing model, the hydrogels with COS-SA conjugates did not exhibit cytotoxicity and exhibited wound healing activity in a mouse model. These results provide evidence that OA and gelatin hydrogels with a COS-SA conjugate can be successfully used for wound healing applications.