Proximity hybridization-triggered DNA assembly for label-free surface-enhanced Raman spectroscopic bioanalysis

We have developed a versatile label-free surface-enhanced Raman spectroscopic platform for detecting various biotargets via proximity hybridization-triggered DNA assembly based on the 736 cm−1 Raman peak of adenine breathing mode. We initially immobilized the first probe to AuNPs and modified the se...

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Bibliographic Details
Published in:Analytica chimica acta 2020-12, Vol.1139, p.42-49
Main Authors: Liao, Xianjiu, Zhang, Caiyi, Machuki, Jeremiah Ong’achwa, Wen, Xiaoqing, Tang, Qianli, Shi, Hengliang, Gao, Fenglei
Format: Article
Language:English
Subjects:
Adenine
DNA
Gold
Label-free
Metal Nanoparticles
Nucleic Acid Hybridization
Proximity hybridization
Raman
Spectrum Analysis, Raman
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Summary:We have developed a versatile label-free surface-enhanced Raman spectroscopic platform for detecting various biotargets via proximity hybridization-triggered DNA assembly based on the 736 cm−1 Raman peak of adenine breathing mode. We initially immobilized the first probe to AuNPs and modified the second with poly adenine. Presence of target DNA or protein molecules assembled a sandwich complex that brought the poly adenine close to the AuNPs surface, generating Raman signals, that were proportional to target molecule concentration. These approach exhibits high sensitivity, with a detection limit of 5.4 pM, 47 fM, and 0.51 pg/mL for target DNA, thrombin and CEA, respectively. Owing to a one step proximity dependent complex formation, this technique is simple and can be completed within 40 min, making it a promising candidate for point-of-care testing applications. A versatile label-free surface-enhanced Raman spectroscopic platform for detecting various biotargets via proximity hybridization-triggered DNA assembly was developed. [Display omitted] •A novel label-free and Raman dyes-free surface enhanced Raman spectroscopy platform is developed.•The SERS signal produced from the adenine base of the DNA.•The biosensor can be completed within 40 minutes.
ISSN:0003-2670
1873-4324
DOI:10.1016/j.aca.2020.09.030