Enabling anoxic acetate assimilation by electrode-driven respiration in the obligate aerobe, Pseudomonas putida

[Display omitted] •P. putida consumes acetate under an anoxic CC-MFC operation.•The anode enables the acetate metabolization acting as an electron sink.•Electrode-based respiration significantly lowers the NADH/NAD + ratio.•Acetyl-CoA synthetase activity of CC-MFC is comparable to aerobic operation....

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Published in:Bioelectrochemistry (Amsterdam, Netherlands) Netherlands), 2021-04, Vol.138, p.107690-107690, Article 107690
Main Authors: Mutyala, Sakuntala, Kim, Changman, Song, Young Eun, Khandelwal, Himanshu, Baek, Jiyun, Seol, Eunhee, Oh, You-kwan, Kim, Jung Rae
Format: Article
Language:English
Subjects:
Acetate
Acetic acid
Acetyl CoA synthetase
Aerobic conditions
Anaerobic conditions
Anoxic conditions
Assimilation
Biochemical fuel cells
Circuits
Electrodes
Energy sources
Fuel technology
Microbial fuel cell
Microorganisms
NAD
NADH
NADH/NAD+ ratio
Nicotinamide adenine dinucleotide
Pseudomonas putida
Respiration
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Summary:[Display omitted] •P. putida consumes acetate under an anoxic CC-MFC operation.•The anode enables the acetate metabolization acting as an electron sink.•Electrode-based respiration significantly lowers the NADH/NAD + ratio.•Acetyl-CoA synthetase activity of CC-MFC is comparable to aerobic operation. This study examined the obligate aerobe, Pseudomonas putida, using acetate as the sole carbon and energy source, and respiration via an anode as the terminal electron acceptor under anoxic conditions. P. putida showed significantly different acetate assimilation in a closed-circuit microbial fuel cell (CC-MFC) compared to an open circuit MFC (OC-MFC). More than 72% (2.6 mmol) of acetate was consumed during 84 hrs in the CC-MFC in contrast to the no acetate consumption observed in the OC-MFC. The CC-MFC produced 150 μA (87 C) from acetate metabolization. Electrode-based respiration reduced the NADH/NAD+ ratio anaerobically, which is similar to the aerobic condition. The CC-MFC showed significantly higher acetyl-CoA synthetase activity than the OC-MFC (0.028 vs. 0.001 μmol/min/mg), which was comparable to the aerobic condition (circa 60%). Overall, electrode-based respiration enables P. putida to metabolize acetate under anoxic conditions and provide a platform to regulate the bacterial redox balance without oxygen.
ISSN:1567-5394
1878-562X
DOI:10.1016/j.bioelechem.2020.107690