Diagnostic, prognostic and predictive values of miR-100 and miR-210 in pediatric acute lymphoblastic Leukemia

• Published in: Hematology (Luxembourg) 2020-01, Vol.25 (1), p.405-413
• Main Authors: Hassan, Naglaa M., Refaat, Lobna A., Ismail, Ghada N., Abdellateif, Mona, Fadel, Sayed A., AbdelAziz, Rania S.
• Format: Article
• Language: English
• Subjects: Adolescent; ALL; Bone Marrow - metabolism; Bone Marrow - pathology; Child; Child, Preschool; Disease-Free Survival; Down-Regulation; Female; Gene Expression Regulation, Leukemic; Humans; Infant; Leukemia; Male; MicroRNAs; MicroRNAs - biosynthesis; miR-100; miR-210; Precursor Cell Lymphoblastic Leukemia-Lymphoma - diagnosis; Precursor Cell Lymphoblastic Leukemia-Lymphoma - metabolism; Precursor Cell Lymphoblastic Leukemia-Lymphoma - mortality; Precursor Cell Lymphoblastic Leukemia-Lymphoma - pathology; Predictive Value of Tests; RNA, Neoplasm - biosynthesis; Survival Rate
• ISSN: 1607-8454; 1607-8454
• DOI: 10.1080/16078454.2020.1843753

: microRNAs are playing important roles in the diagnosis and prognosis of pediatric acute lymphoblastic leukemia (ALL). Expression levels of miR-100 and miR-210 were assessed in bone marrow aspirate of 85 pediatric ALL patients compared to 12 healthy control using quantitative real-time polymerase chain reaction. Data were correlated with relevant clinico-pathological features of the patients, response to treatment, disease-free survival (DFS), and overall survival (OS). miR-100 was significantly downregulated in ALL patients [median: 1.21, range: 0-434.3] compared to the control group [median: 8.41, range; 0-840.3, P = 0.035]. miR-210 was significantly upregulated in ALL patients [median: 6.34, range: 1.16-1088.7] compared to the control group [median: 2.57, range: 0.11-709.2, P  = 0.025]. The sensitivity, specificity, and area under curve of miR-100 were (64.7%, 62.5%, and 0.642; respectively, P = 0.035) at a cut-off 2.6 and that of miR-210 were (60%, 58.3% and 0.650; respectively, P = 0.025) at a cut-off 3.5. miR-100 overexpression associated with shorter DFS and OS (P = 0.033 and 0.046; respectively). Patients with miR-100 lowexpression showed a significant incidence of late death ( P = 0.024). There was no significant association between miR-210 expression and DFS, OS, incidence of early or late death. : miR-100 and miR-210 could be used as potential diagnostic markers for pediatric ALL. miR-100 is a useful prognostic and predictive biomarker for childhood ALL.