Structure and gene cluster of the O-antigen of Escherichia coli strain SDLZB008

The O-polysaccharide (O-antigen) of Escherichia coli SDLZB008 was isolated from the lipopolysaccharide and studied by sugar analyses along with 1H and 13C NMR spectroscopy. The following structure of the branched pentasaccharide repeating unit was established, which is unique among the known structu...

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Bibliographic Details
Published in:Carbohydrate research 2020-12, Vol.498, p.108154-108154, Article 108154
Main Authors: Perepelov, Andrei V., Song, Yajun, Zhu, Yiming, Shashkov, Alexander S., Filatov, Andrei V., Hu, Bin
Format: Article
Language:English
Subjects:
Bacterial polysaccharide structure
Escherichia coli
Lipopolysaccharide
O-antigen
O-antigen gene cluster
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Summary:The O-polysaccharide (O-antigen) of Escherichia coli SDLZB008 was isolated from the lipopolysaccharide and studied by sugar analyses along with 1H and 13C NMR spectroscopy. The following structure of the branched pentasaccharide repeating unit was established, which is unique among the known structures of bacterial polysaccharides:▪ The O-antigen gene cluster of E. coli SDLZB008 has been sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in full agreement with the O-polysaccharide structure. [Display omitted] •Strain SDLZB008 is a new strain of E. coli.•The OPS structure of E. coli SDLZB008 was established.•The OPS was found to contain d-Fucp3NAc residue.•The O-antigen gene cluster of E. coli SDLZB008 was sequenced and annotated.
ISSN:0008-6215
1873-426X
DOI:10.1016/j.carres.2020.108154