Investigation of intestinal transportation of peptide‐displaying bacteriophage particles using phage display method

• Published in: Journal of peptide science 2021-03, Vol.27 (3), p.e3292-n/a
• Main Authors: Kanaani, Hakimeh, Azarmi, Yadollah, Dastmalchi, Siavoush, Zarei, Omid, Hamzeh‐Mivehroud, Maryam
• Format: Article
• Language: English
• Subjects: Amino acids; Bioinformatics; Drug delivery; gastrointestinal barrier; in vivo biopanning; Intestine; Libraries; Mucosa; Oral administration; peptide library; Peptides; Phage display; Phages; Software; Translocation
• ISSN: 1075-2617; 1099-1387
• DOI: 10.1002/psc.3292

To investigate whether peptide sequences with specific translocation across the gastrointestinal barrier can be identified as drug delivery vehicles, in vivo phage display was conducted. For this purpose, a random library of 12‐mer peptides displayed on M13 bacteriophage was orally administered to mice followed by recovery of the phage particles from the blood samples after three consecutive biopanning rounds. The obtained peptide sequences were analyzed using bioinformatics tools and software. The results demonstrated that M13 bacteriophage bearing peptides translocate nonspecifically across the mice intestinal mucosal barrier deduced from random distribution of amino acids in different positions of the identified peptide sequences. The most probable reason for entering the phage particles into systemic circulation after oral administration of the peptide library can be related to the nanoscale nature of their structures which provides a satisfying platform for the purpose of designing nanocarriers in pharmaceutical applications. In the present study, phage display technique was applied for identification of novel 12‐mer peptide‐based drug delivery vehicles using peptide library in in vivo platform. The findings indicated that phage particles can pass the intestinal barrier and enter to the systemic circulation following administration by gavage in mice. The results demonstrated that M13 bacteriophage bearing peptides translocate nonspecifically across the mice intestinal mucosal barrier deduced from random distribution of amino acids in different positions of the identified peptide sequences.