Paracrine effect of human adipose-derived stem cells on lymphatic endothelial cells

The proposal of this study was to evaluate, , the potential paracrine effect of human adipose-derived stem cells (hASCs) to promote lymphangiogenesis in lymphatic endothelial cells isolated from rat diaphragmatic lymphatic vessels.  ELISA on VEGFA, VEGFC and IL6 in hASC-conditioned medium; LYVE1 imm...

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Bibliographic Details
Published in:Regenerative medicine 2020-09, Vol.15 (9), p.2085-2098
Main Authors: Marcozzi, Cristiana, Frattini, Annalisa, Borgese, Marina, Rossi, Federica, Barone, Ludovica, Solari, Eleonora, Valli, Roberto, Gornati, Rosalba
Format: Article
Language:English
Subjects:
conditioned medium
ELISA
gene expression
immunohistochemistry
lymphangiogenesis
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Summary:The proposal of this study was to evaluate, , the potential paracrine effect of human adipose-derived stem cells (hASCs) to promote lymphangiogenesis in lymphatic endothelial cells isolated from rat diaphragmatic lymphatic vessels.  ELISA on VEGFA, VEGFC and IL6 in hASC-conditioned medium; LYVE1 immunostaining; and gene expression of , , and were the methods used.  In 2D culture, hASC-conditioned medium was able to promote lymphatic endothelial cell survival, maintenance of endothelial cobblestone morphology and induction to form a vessel-like structure.  The authors' results represent evidence of the paracrine effect of hASCs on lymphatic endothelial cells, suggesting the possible role of hASC-conditioned medium in developing new therapeutic approaches for lymphatic system-related dysfunction such as secondary lymphedema. This work is addressed to provide, by scientific experiments, information about the possibility of correcting localized swelling of the body caused by an abnormal accumulation of lymph using culture medium enriched with metabolites, growth factors and extracellular matrix proteins secreted by a stem cell population. The authors' results could be an innovative therapeutic approach for regenerative medicine and may have an important impact on the treatment of lymphatic-related pathologies.
ISSN:1746-0751
1746-076X
DOI:10.2217/rme-2020-0071