Glyphidohaptor safiensis n. sp. (Monogenea: Ancyrocephalidae) from the white-spotted rabbitfish Siganus canaliculatus (Park) (Perciformes: Siganidae) off Oman, with notes on its phylogenetic position within the Ancyrocephalidae Bychowsky & Nagibina, 1968 (sensu lato)

• Published in: Systematic parasitology 2020-12, Vol.97 (6), p.727-741
• Main Authors: Al Jufaili, Sarah H., Machkevsky, Vladimir K., Al Kindi, Umkulthoum H., Palm, Harry W.
• Format: Article
• Language: English
• Subjects: Agriculture; Ancyrocephalidae; Animal Anatomy; Animal Ecology; Animal Systematics/Taxonomy/Biogeography; Animals; Biomedical and Life Sciences; Congeners; DNA, Ribosomal - genetics; Fossils; Gills; Histology; Indian Ocean; Life Sciences; Monogenea; Morphology; New records; New species; Oman; Perciformes - parasitology; Phylogenetics; Phylogeny; Pseudohaliotrema; Shells; Siganus canaliculatus; Spacer region; Species Specificity; Tetrancistrum; Trematoda - anatomy & histology; Trematoda - classification; Trematoda - genetics; Zoology
• ISSN: 0165-5752; 1573-5192
• DOI: 10.1007/s11230-020-09949-x

A new ancyrocephalid monogenean is described from the gills of wild White-spottedrabbitfish Siganus canaliculatus (Park) based on morphological and molecular analyses. Glyphidohaptor safiensis n. sp. can be distinguished from other members of the genus by the shape of the accessory piece of the male copulatory organ (MCO). Unlike its congeners, the rod-shaped accessory piece of G. safiensis n. sp. is distally broad and flattened. The MCO of G. safiensis n. sp. is curved, enclosed in a heavy sheath with a terminal flap. Partial large subunit (LSU), partial small subunit (SSU) and the partial SSU, entire internal transcribed spacer region 1 (ITS1) and partial 5.8S rDNA of the new species and two species of Tetrancistrum Goto & Kikuchi, 1917 from the same host and locality were sequenced and subjected to phylogenetic analysis. The LSU rDNA analysis grouped G. safiensis n. sp. with Tetrancistrum sp. from the gills of Siganus fuscescens Houttuyn from Australia, indicating a possible misidentification of the latter. Sequences of the SSU rDNA of the new species were most similar to those for Pseudohaliotrema sphincteroporus Yamaguti, 1953, demonstrating the close relatedness of the genera Pseudohaliotrema Yamaguti, 1953 and Glyphidohaptor Kritsky, Galli & Yang, 2007 within the Ancyrocephalidae. The comparison of the partial SSU (424 bp) and entire ITS1 and partial 5.8S rDNA (246 bp) sequences obtained for G. safiensis n. sp. with the only available sequence of another member of Glyphidohaptor Kritsky, Galli & Yang, 2007, G. pletocirra Paperna, 1972 (HE601931-HE601933) yielded on average 1.08% dissimilarity (a difference of 7 bases), with a p-distance of 0.010 ± 0.004%. This is the first record of a species of Glyphidohaptor from S. canaliculatus and from the Persian Gulf, the Gulf of Oman and the Arabian Sea.