The effect of emodin on melanogenesis through the modulation of ERK and MITF signaling pathway

The aim of this study was to investigate the effect of emodin derived from on melanin production in B16F1 cells. In this study, emodin did not show antioxidant activity in DPPH radical and reducing power assays. However, it was found that emodin scavenged intracellular H O . Emodin increased not onl...

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Bibliographic Details
Published in:Natural product research 2022-02, Vol.36 (4), p.1084-1088
Main Authors: Kim, Jaeho, Kim, Moon-Moo
Format: Article
Language:English
Subjects:
Animals
Antioxidants
Aquatic plants
Cell Line, Tumor
Emodin
Emodin - pharmacology
Extracellular signal-regulated kinase
Extracellular Signal-Regulated MAP Kinases
Fallopia multiflora - chemistry
FOXO1 protein
Hydrogen peroxide
Hydrogen Peroxide - pharmacology
Immunofluorescence
Melanin
Melanins - biosynthesis
Melanoma, Experimental
Microphthalmia-Associated Transcription Factor
Monophenol Monooxygenase - metabolism
Proteins
Signal transduction
Signal Transduction - drug effects
SIRT1 protein
Synthesis
Transcription activation
Tyrosinase
Tyrosine
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Summary:The aim of this study was to investigate the effect of emodin derived from on melanin production in B16F1 cells. In this study, emodin did not show antioxidant activity in DPPH radical and reducing power assays. However, it was found that emodin scavenged intracellular H O . Emodin increased not only tyrosinase activity but also melanin synthesis in vitro. Moreover, emodin enhanced melanin synthesis by increasing the expression level of tyrosinase (TYR), tyrosine related protein (TRP)-1, TRP-2, MITF and SIRT1 proteins in live cells treated with H O compared with H O treatment group in the analyses of western blot and immunofluorescence. Moreover, emodin suppressed ERK activation by SIRT1 and FOXO1. Thus, emodin promoted melanin synthesis by increasing the expression of TRP-1, TRP-2, tyrosinase through the activation of MITF transcription factor. These findings suggest that emodin could promote melanin production related to anti-hair graying.
ISSN:1478-6419
1478-6427
DOI:10.1080/14786419.2020.1849200