Bacterial cellulose as an oleaginous yeast cell carrier for soybean oil refinery effluent treatment and pyrolysis oil production

• Published in: Bioprocess and biosystems engineering 2021-04, Vol.44 (4), p.661-671
• Main Authors: Qiao, Nan, Fan, Xue, Hu, Shuang, Zhang, Xiuzhen, Wang, Ling, Du, Yundi, Wang, Lei, Zhang, Xiaojun, Yu, Dayu
• Format: Article
• Language: English
• Subjects: Alkanes; Bacteria; Bacteria - metabolism; Biocompatibility; Biological Oxygen Demand Analysis; Biotechnology; Calorific value; Cellulose; Cellulose - chemistry; Chemical oxygen demand; Chemistry; Chemistry and Materials Science; Culture Media; Effluent treatment; Effluents; Environmental Engineering/Biotechnology; Fermentation; Food Science; Glucose - chemistry; Glycine max - metabolism; Immobilization; Industrial and Production Engineering; Industrial Chemistry/Chemical Engineering; Industrial Waste; Microscopy, Electron, Scanning; Oil and Gas Industry; Peptones - chemistry; Petroleum refineries; Pyrolysis; Pyrolysis products; Refineries; Research Paper; Soybean oil; Soybeans; Temperature; Thermogravimetry; Vegetable oils; Waste Disposal, Fluid - instrumentation; Waste Disposal, Fluid - methods; Wastewater treatment; Water Pollutants, Chemical - isolation & purification; Water Purification - instrumentation; Water Purification - methods; Water treatment; Yeast; Yeasts
• ISSN: 1615-7591; 1615-7605
• DOI: 10.1007/s00449-020-02476-5

Bacterial cellulose produced from soybean oil refinery effluent is a good immobilization carrier because of the large pores in its fiber network, its high water-holding capacity, and its good biocompatibility. In this study, it was applied to immobilization of oleaginous yeasts for treating soybean oil refinery effluent. The immobilization percentage reached 50%, and the removal of chemical oxygen demand and oil content reached 92.1% and 93.1%, respectively, during dynamic immobilization using a mass percentage of bacterial cellulose of 30% and an immobilization time of 24 h, which were significantly higher than those of free oleaginous yeasts or yeasts immobilized by bacterial cellulose from rich medium. The immobilized oleaginous yeasts facilitated the recovery of the yeasts and effectively treated three batches of soybean oil refinery effluent. The immobilized oleaginous yeasts recovered after soybean oil refinery effluent treatment were pyrolyzed to produce bio-oil, which contributed to more alkanes and a higher calorific value of bio-oil in the pyrolysis products as compared to those of free oleaginous yeasts. As bacterial cellulose used as an oleaginous yeast cell carrier is produced from soybean oil refinery effluent, no waste of immobilization materials is involved and an efficient waste-into-oil bioprocess is developed.